Annexin V (FITC) / PI Apoptosis Detection Kit
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Description
Annexin V is used as a probe to detect cells that have expressed phosphatidylserine (PS) on the cell surface, an event found in apoptosis as well as other forms of cell death. The Annexin V affinity assay typically uses a conjugate of annexin V and a fluorescent or enzymatic label, biotin or other tags, or a radioelement, in a suitable buffer (Annexin V binding to PS is Ca2+ dependent). The assay combines Annexin V staining of PS membrane events with the staining of the cell nucleus with PI or AAD-7 to distinguish living cells from dead cells. Annexin V apoptosis detection is based on the observation that soon after initiating apoptosis, cells translocate the membrane phosphatidylserine (PS) from the inner (cytoplasmic-facing) leaflet of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for PS. Detection can be analyzed by flow cytometry or by fluorescence microscopy.
Documents del producto
Product specifications
| Category | Assay Kits |
| Immunogen Target | Annexin V |
| Reactivity | General |
| Conjugation | FITC |
| Size 1 | 100 tests |
| Size 2 | 200 tests |
| Buffer | 11 ml (Annexin V Binding Buffer (10X) |
| Availability | Shipped within 5-10 working days. |
| Storage | Store at at 2-8°C in the dark. |
| Dry Ice | No |
| Status | RUO |
| Note | NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. The validity for this kit is 12 months. Excitation/Emission: 499/515 Laser line: 488 |
Descripción
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