Intestinal-Type Alkaline Phosphatase / IAP (ALPI) Antibody
292.5€ (80 µl)
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Name
Intestinal-Type Alkaline Phosphatase / IAP (ALPI) Antibody
Category
Primary Antibodies
Provider
Abbexa
Reference
abx028323
Tested Applications
ELISA, WB, IHC, IF/ICC
Description
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is upregulated during small intestinal epithelial cell differentiation.
Documentos del producto
Instrucciones
Data sheet
Especificaciones del producto
| Category | Primary Antibodies |
| Immunogen Target | Target: Intestinal-Type Alkaline Phosphatase / IAP (ALPI) Immunogen: KLH-conjugated synthetic peptide between 274-304 amino acids from the Central region of human Alkaline Phosphatase (ALPI). |
| Host | Rabbit |
| Reactivity | Human |
| Recommended Dilution | WB: 1/1000, IHC-P: 1/100, IF/ICC: 1/100. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user. |
| Clonality | Polyclonal |
| Conjugation | Unconjugated |
| Isotype | IgG |
| Observed MW | Calculated MW: 56.8 kDa |
| Purification | Purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
| Size 1 | 80 µl |
| Size 2 | 400 µl |
| Form | Liquid |
| Tested Applications | ELISA, WB, IHC, IF/ICC |
| Buffer | PBS containing 0.09% sodium azide. |
| Availability | Shipped within 5-10 working days. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| Dry Ice | No |
| UniProt ID | P09923 |
| NCBI Accession | NP_001622.2 |
| Alias | IAP |
| Background | Antibody anti-ALPI |
| Status | RUO |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |
Background
Intestinal-type alkaline phosphatase (ALPI) is a glycoprotein enzyme primarily expressed on the luminal surface of intestinal epithelial cells, particularly in the small intestine. It plays a crucial role in maintaining gut homeostasis by detoxifying lipopolysaccharides (LPS), dephosphorylating inflammatory mediators, and promoting a healthy microbiota. ALPI is also involved in nutrient absorption, such as the hydrolysis of dietary phosphate esters, contributing to the regulation of phosphate metabolism. Its expression is upregulated in response to inflammation and microbial imbalance, acting as a protective mechanism against intestinal permeability and systemic endotoxemia. Additionally, ALPI prevents excessive immune activation by neutralizing pro-inflammatory signals, which is essential for preventing conditions such as inflammatory bowel disease (IBD) and sepsis. Alterations in ALPI expression or activity have been linked to gastrointestinal disorders, including Crohn's disease and ulcerative colitis. Its multifunctional role highlights its importance in gut health, immune modulation, and overall metabolic regulation.
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