ALK Tyrosine Kinase Receptor (ALK) Cell ELISA Kit
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Description
ALK Cell ELISA Kit is a cell-based ELISA Kit. Cells to be assayed should be seeded onto a clear flat bottom 96 well plate, using poly-L-lysine for non-adherent cells. Cells should be grown to 75-90% confluence and treated prior to carrying out the ELISA.
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Product specifications
| Category | ELISA Kits |
| Immunogen Target | ALK Tyrosine Kinase Receptor (ALK) |
| Reactivity | Human, Mouse |
| Detection Method | Colorimetric |
| Recommended Dilution | Optimal dilutions/concentrations should be determined by the end user. |
| Size 1 | 96 tests |
| Tested Applications | ELISA |
| Availability | Shipped within 1-2 weeks. The validity for this kit is at least 6 months |
| Storage | Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual. |
| Dry Ice | No |
| UniProt ID | Q9UM73 |
| Gene ID | 238 |
| OMIM | 105590 |
| Alias | CD246 |
| Background | Elisa kits for ALK |
| Status | RUO |
| Note | Validity: 6 months. This product is for research use only. The range and sensitivity is subject to change. Please contact us for the latest product information. For accurate results, sample concentrations must be diluted to mid-range of the kit. If you require a specific range, please contact us in advance or write your request in your order comments. Please note that our ELISA and CLIA kits are optimised for detection of native samples, rather than recombinant proteins. We are unable to guarantee detection of recombinant proteins, as they may have different sequences or tertiary structures to the native protein. |
Descripción
The ALK (anaplastic lymphoma kinase) gene encodes a receptor tyrosine kinase, which is a type of cell surface receptor that plays a role in signaling pathways involved in cell growth, proliferation, and differentiation. The ALK protein belongs to the insulin receptor superfamily and is normally expressed in specific tissues during embryonic development, where it helps regulate normal cell growth and development. While ALK expression is typically limited to certain tissues during development, aberrant activation of the ALK gene can occur in various cancers due to chromosomal rearrangements, gene amplification, or activating mutations. These alterations lead to the overexpression or constitutive activation of the ALK protein, contributing to oncogenesis (the formation of cancer).The most well-known oncogenic alteration involving the ALK gene is the fusion of the ALK gene with another gene, resulting in a chimeric fusion protein with constitutive kinase activity. This fusion protein can drive cellular transformation and tumorigenesis.
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ALK antibody
Anaplastic Lymphoma Kinase (ALK), also known as CD246, is a 220 kDa transmembrane glycoprotein that is primarily expressed in the developing nervous system. ALK contains one LDL Receptor (LDLR) class A domain, two meprin/A5-protein/PTPmu (MAM) domains, and a cytoplasmic kinase domain. Chromosomal translocation involving ALK and the nucleolar NPM protein result in a cytoplasmic fusion protein that exists as a dimer. The kinase domain in NPM-ALK is constitutively active and participates in cellular transformation. ALK translocations are found in a variety of tumors. Within the extracellular domain, human and mouse ALK share 88% amino acid sequence identity.
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