Acetyl-CoA Carboxylase 1 (ACACA) Cell ELISA Kit
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Description
ACC1 Cell ELISA Kit is a cell-based ELISA Kit. Cells to be assayed should be seeded onto a clear flat bottom 96 well plate, using poly-L-lysine for non-adherent cells. Cells should be grown to 75-90% confluence and treated prior to carrying out the ELISA.
Documents del producto
Product specifications
| Category | ELISA Kits |
| Immunogen Target | Acetyl-CoA Carboxylase 1 (ACACA) |
| Reactivity | Human, Mouse, Rat |
| Detection Method | Colorimetric |
| Recommended Dilution | Optimal dilutions/concentrations should be determined by the end user. |
| Size 1 | 96 tests |
| Tested Applications | ELISA |
| Availability | Shipped within 1-2 weeks. The validity for this kit is at least 6 months |
| Storage | Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual. |
| Dry Ice | No |
| UniProt ID | Q13085 |
| Gene ID | 31 |
| OMIM | 200350 |
| Alias | ACACD,ACACalpha,ACC,ACC1,ACCA |
| Background | Elisa kits for ACACA |
| Status | RUO |
| Note | Validity: 6 months. This product is for research use only. The range and sensitivity is subject to change. Please contact us for the latest product information. For accurate results, sample concentrations must be diluted to mid-range of the kit. If you require a specific range, please contact us in advance or write your request in your order comments. Please note that our ELISA and CLIA kits are optimised for detection of native samples, rather than recombinant proteins. We are unable to guarantee detection of recombinant proteins, as they may have different sequences or tertiary structures to the native protein. |
Descripción
Acetyl Coenzyme A Carboxylase Alpha (ACACA) is a cytosolic enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, a critical step in fatty acid biosynthesis. ACACA is the rate-limiting enzyme in the synthesis of long-chain fatty acids and plays a central role in lipogenesis. It is highly expressed in lipogenic tissues such as the liver and adipose tissue and is tightly regulated by phosphorylation, dephosphorylation, and allosteric effectors like citrate and palmitoyl-CoA. Dysregulation of ACACA activity contributes to metabolic disorders such as obesity, type 2 diabetes, and fatty liver disease, where excessive lipogenesis exacerbates lipid accumulation. In cancer, ACACA is upregulated to support the enhanced lipid biosynthesis required for rapid tumor cell proliferation, making it a potential therapeutic target. Small-molecule inhibitors of ACACA are being developed to treat metabolic diseases and cancer by reducing de novo fatty acid synthesis.
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