5'-Nucleotidase / CD73 (NT5E) Antibody
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Description
NT5E Antibody is a Rabbit Polyclonal antibody against NT5E. The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.
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Product specifications
| Category | Primary Antibodies |
| Immunogen Target | 5'-Nucleotidase / CD73 (NT5E) |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Recommended Dilution | WB: 1/500 - 1/2000, IHC-P: 1/50 - 1/200. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user. |
| Clonality | Polyclonal |
| Conjugation | Unconjugated |
| Isotype | IgG |
| Purification | Purified by affinity chromatography. |
| Size 1 | 60 µl |
| Size 2 | 120 µl |
| Size 3 | 200 µl |
| Form | Liquid |
| Tested Applications | WB, IHC |
| Buffer | PBS, pH 7.3, containing 0.01% thiomersal, 50% glycerol. |
| Availability | Shipped within 5-10 working days. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| Dry Ice | No |
| UniProt ID | P21589 |
| Gene ID | 4907 |
| NCBI Accession | NP_002517.1 |
| Alias | NT,En,NT5,NTE,eNT,CD73,E5NT,CALJA,5'-NT,5'-deoxynucleotidase,Ecto-5'-nucleotidase,IMP-specific 5'-nucleotidase,Thymidylate 5'-phosphatase |
| Background | Antibody anti-NT5E |
| Status | RUO |
| Note | Concentration: 1 mg/ml - |
Descripción
The NT5E gene encodes 5'-nucleotidase ecto, also known as CD73, a glycosylphosphatidylinositol (GPI)-anchored enzyme that is crucial in purinergic signaling. It catalyzes the hydrolysis of extracellular nucleotides into nucleosides, with a primary function of converting AMP (adenosine monophosphate) to adenosine. CD73 is expressed on a wide range of cell types, including endothelial cells, epithelial cells, fibroblasts, T cells, and tumor cells. It plays a fundamental role in regulating inflammation, vascular permeability, immune response, and tissue protection under stress conditions such as ischemia. Adenosine generated by CD73 is a potent signaling molecule that binds to adenosine receptors (A1, A2A, A2B, and A3) to mediate various physiological processes, including anti-inflammatory effects, vasodilation, and immunosuppression. In the immune system, CD73 suppresses pro-inflammatory responses and helps in the resolution of inflammation. This dual role makes NT5E a key molecule in both health and disease, especially in pathologies such as cancer, cardiovascular diseases, and chronic inflammatory disorders.
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anti- NT5E/CD73 antibody
NT5E(5'-nucleotidase) is also named as NT5, NTE,CD73,ecto-5'-nucleotidase, and belongs to the 5'-nucleotidase family. It catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP.The enzyme occurs mainly as a dimer and the wide distribution of NT5E in the rat hippocampus, suggesting their involvement in the control of the purinergic signaling(PMID:17619139). This protein has two isoforms with the molecular weight of 63 kDa and 58 kDa and four glycosylation sites with a signalpeptide. It can exsit as a dimer(PMID:17619139). It can be detected the band of 55 kDa,64 kDa, 70 kDa, 110 kDa by western blot(PMID:17619139; 12370277; 21533188). Defects in NT5E are the cause of calcification of joints and arteries(CALJA).
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