2-MET Urine RIA Fast Track

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Precio

Precio a consultar

proveedor

LDN

reference

BA R-8600

Tested Applications

RIA

reactivity

human

status

CE Marked

clonality

Descripción

Fast Track radioimmunoassay (RIA) for the quantitative determination of metanephrine and normetanephrine in urine.


Fast Track radioimmunoassay (RIA) para la determinación cuantitativa de metanefrina y normetanefrina en orina.

Background

125I – Radioimmunoassay for the quantitative determination of metanephrine and normetanephrine in urine. First, metanephrine (metadrenaline) and normetanephrine (normetadrenaline) are quantitatively acylated. The assay procedure follows the basic principle of radioimmunoassay, involving competition between a radioactive and a non-radioactive antigen for a fixed number of antibody binding sites. The amount of 125Ilabelled antigen bound to the antibody is inversely proportional to the analyte concentration of the sample. When the system is in equilibrium, the antibody bound radioactivity is precipitated with a second antibody in the presence of polyethylene glycol. The precipitate is counted in a gamma counter. Quantification of unknown samples is achieved by comparing their activity with a standard curve prepared with known standards


Radioinmunoensayo 125I para la determinación cuantitativa de metanefrina y normetanefrina en la orina. Primero, la metanefrina (metadrenalina) y la normetanefrina (normetadrenalina) se acilan cuantitativamente. El procedimiento del ensayo sigue el principio básico del radioinmunoensayo, que implica la competencia entre un antígeno radiactivo y un antígeno no radiactivo por un número fijo de sitios de unión de anticuerpos. La cantidad de antígeno marcado con 125I unido al anticuerpo es inversamente proporcional a la concentración del analito en la muestra. Cuando el sistema está en equilibrio, la radiactividad unida al anticuerpo se precipita con un segundo anticuerpo en presencia de polietilenglicol. El precipitado se cuenta en un contador gamma. La cuantificación de muestras desconocidas se logra comparando su actividad con una curva estándar preparada con estándares conocidos.

Características del producto

category

RIA Kits


size 1

2 x 100 det.


storage

2 – 8 °C


test range

Metanephrine: 0 / 20 – 2 000 ng/mlNormetanephrine: 0 / 30 – 3 000 ng/ml


detection method

Common sample preparation. Acylation in liquid phase. 6 standards. 2 controls. Ready for use. Double antibody technique.


assay data

Hydrolysis and acylation 45 min; RIA 1 hour 30 min


availability

Inmediate


dry ice

No


note

25 µl urine


alias

Metanephrine, Normetanephrine, Metanefrina,Normetanefrina,Metanefrine


reseach area

Metanephrine and Normetanephrine in Urine


status

CE Marked


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