2-MET Urine ELISA Fast Track

Background

Enzyme Immunoassay for the quantitative determination of Metanephrine and Normetanephrine in urine. During the sample preparation Metanephrine (Metadrenaline) and Normetanephrine (Normetadrenaline) are quantitatively acylated. The subsequent competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples and the solid phase bound analytes compete for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm

Background

Inmunoensayo enzimático para la determinación cuantitativa de Metanefrina y Normetanefrina en la orina. Durante la preparación de la muestra, la Metanefrina (Metadrenalina) y la Normetanefrina (Normetadrenalina) se acilan cuantitativamente. El kit de ELISA competitivo posterior utiliza el formato de microplaca. El antígeno se une a la fase sólida de la microplaca. Los estándares acilados, los controles y las muestras, así como los analitos unidos a la fase sólida, compiten por un número fijo de sitios de unión de anticuerpos. Después de que el sistema esté en equilibrio, el antígeno libre y los complejos antígeno-anticuerpo libres se eliminan mediante lavado. El anticuerpo unido a la fase sólida se detecta mediante un conjugado de anti-IgG-peroxidasa (Cconejo) utilizando TMB como sustrato. La reacción se lee a 450 nm.

Características del producto

reactivity

human

test range

Metanephrine: 0 / 20 – 2 000 ng/mlNormetanephrine: 0 / 30 – 3 000 ng/mlSensitivity:

assay data

Hydrolysis and acylation 45 min; ELISA 1 hour

detection method

Common sample preparation. Acylation in liquid phase. 6 standards. 2 controls. Ready for use.

size 1

2 x 96 det

alias

Metanephrine, Normetanephrine, Metanefrina,Normetanefrina,Metanefrine

storage

2 – 8 °C

or code

Metanephrine and Normetanephrine in Urine

dry ice

note

25 µl urine

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Metanephrine Urine ELISA Fast Track

proveedor

LDN

reference

BA E-8400

Tested Applications

Elisa

reactivity

human

status

CE Marked

Fast Track enzyme immunoassay (ELISA) for the quantitative determination of metanephrine in urine.

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Normetanephrine Urine ELISA Fast Track

proveedor

LDN

reference

BA E-8500

Tested Applications

Elisa

reactivity

human

status

CE Marked

Fast Track enzyme immunoassay (ELISA) for the quantitative determination of normetanephrine in urine.

Precio a consultar