BIK - BCL2 Interacting Killer | Elisa - Clia - Antibody - Protein
Family main features
Background
BCL2 Interacting Killer (BIK), also known as NBK (Nineteen-kilodalton Bcl-2-interacting Killer), is a pro-apoptotic protein that belongs to the Bcl-2 family. The Bcl-2 family of proteins plays a pivotal role in the regulation of apoptosis, with members classified into anti-apoptotic and pro-apoptotic groups based on their function. BIK, identified through its interaction with Bcl-2, is known to promote apoptosis by antagonizing the function of anti-apoptotic proteins like Bcl-2 and Bcl-XL. Its expression is regulated by various stimuli, including DNA damage, oncogene activation, and growth factor deprivation.
Protein Structure
BIK is characterized by several structural features typical of Bcl-2 family proteins:
- BH3 Domain: The BH3 (Bcl-2 homology 3) domain is crucial for its pro-apoptotic function, allowing BIK to interact with and neutralize anti-apoptotic Bcl-2 family members.
- Hydrophobic C-Terminus: This region helps BIK anchor to intracellular membranes, particularly the mitochondria and endoplasmic reticulum (ER), where it exerts its pro-apoptotic effects.
- N-Terminal Domain: Contains sites for post-translational modifications, such as phosphorylation, which can regulate BIK's activity and stability.
Classification and Subtypes
BIK is classified as a BH3-only protein within the Bcl-2 family, which includes other pro-apoptotic proteins such as BID, BAD, and NOXA. These proteins share the BH3 domain but lack other Bcl-2 homology domains found in multi-domain pro-apoptotic proteins like BAX and BAK.
Function and Biological Significance
BIK plays a significant role in promoting apoptosis through several mechanisms:
- Mitochondrial Pathway Activation: BIK induces mitochondrial outer membrane permeabilization (MOMP), leading to the release of cytochrome c and subsequent activation of caspases, which are essential for the execution phase of apoptosis.
- ER Stress and Apoptosis: BIK can localize to the ER and contribute to apoptosis in response to ER stress by modulating calcium homeostasis and interacting with other apoptotic regulators.
- Interaction with Anti-Apoptotic Proteins: BIK binds to anti-apoptotic proteins like Bcl-2 and Bcl-XL through its BH3 domain, inhibiting their function and promoting cell death.
Clinical Issues
- Cancer: Dysregulation of apoptosis is a hallmark of cancer, and alterations in the expression or function of BIK can contribute to tumorigenesis. In some cancers, BIK expression is downregulated or its apoptotic function is inhibited, allowing cancer cells to evade programmed cell death.
- Therapeutic Potential: Restoring BIK function or mimicking its activity with BH3 mimetics represents a potential therapeutic strategy for inducing apoptosis in cancer cells resistant to conventional therapies.
- Drug Resistance: Some studies suggest that BIK may be involved in the sensitivity of cancer cells to chemotherapeutic agents, making it a potential marker for predicting treatment response.
Summary
BIK (BCL2 Interacting Killer) is a pro-apoptotic BH3-only protein that plays a critical role in regulating apoptosis. By antagonizing anti-apoptotic Bcl-2 family members and promoting mitochondrial dysfunction, BIK is essential for programmed cell death in response to various cellular stresses. Its dysregulation is implicated in cancer progression and resistance to therapy, highlighting its potential as a target for cancer treatment. Understanding the precise mechanisms by which BIK induces apoptosis and its interactions with other apoptotic regulators is crucial for developing novel therapeutic approaches to enhance cell death in cancerous cells.
BIK Recommended name:
BCL2 Interacting Killer (BIK)
Aliases for BIK
BIP1,BP4,NBK,Apoptosis inducer NBK
En la tabla siguiente se muestra una comparativa de todos los reactivos disponibles en nuestro catálogo (Primary Antibodies, ELISA Kits) relacionados con BIK - BCL2 Interacting Killer
Se muestran ordenados por categorías para poder comparar cómodamente sus características principales. Esta tabla, que contiene un enlace con la ficha de cada producto, es exportable a Excel.
Esta página contiene 9 reactivos de las marcas (Abbexa, FineTest) que se corresponden con tu busqueda
Contacta con nosotros en info@markelab.com, si necesitas mas informacion o alguna aclaracion. Te garantizamos respuesta en menos de 24 h.
immunoassays
provider | Code | reference | name | reactivity | sample type | assay type | test range | sensitivity | price | size 1 | uniprot id | status |
---|---|---|---|---|---|---|---|---|---|---|---|---|
Abbexa | BIK | abx595063 | BIK Cell ELISA Kit | Human | Cell ELISA | 493.75 | 96 tests | Q13323 | RUO |
Primary Antibodies
provider | Code | reference | name | reactivity | clonality | host | immunogen target | isotype | conjugation | tested applications | price | size 1 | uniprot id | status |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Abbexa | BIK | abx324388 | BCL2 Interacting Killer Phospho-Thr33 (BIK pT33) Antibody | Human | Polyclonal | Rabbit | BCL2 Interacting Killer Phospho-Thr33 (BIK pT33) | Unconjugated | ELISA, WB, IHC | 187.5 | 50 µg | Q13323 | RUO | |
Abbexa | BIK | abx001932 | Bcl-2-Interacting Killer (BIK) Antibody | Human | Polyclonal | Rabbit | Bcl-2-Interacting Killer (BIK) | Unconjugated | WB | 175 | 20 µl | Q13323 | RUO | |
Abbexa | BIK | abx320215 | Bcl-2-Interacting Killer (BIK) Antibody | Human | Polyclonal | Rabbit | Bcl-2-Interacting Killer (BIK) | Unconjugated | ELISA, IHC | 237.5 | 50 µl | Q13323 | RUO | |
Abbexa | BIK | abx411860 | Bcl-2-Interacting Killer (BIK) Antibody | Human | Polyclonal | Rabbit | Bcl-2-Interacting Killer (BIK) | Unconjugated | WB, IHC | 437.5 | 100 µg | Q13323 | RUO | |
Abbexa | BIK | abx027273 | Bik BH3 Domain Antibody | Human | Polyclonal | Rabbit | Bik BH3 Domain | Unconjugated | ELISA, WB, IHC | 281.25 | 80 µl | Q13323 | RUO | |
Abbexa | BIK | abx324775 | BCL2 Interacting Killer (BIK) Antibody | Human | Polyclonal | Rabbit | BCL2 Interacting Killer (BIK) | Unconjugated | ELISA, WB, IHC, IF/ICC | 187.5 | 50 µg | Q13323 | RUO | |
Abbexa | BIK | abx332949 | BCL2 Interacting Killer Phospho-Thr33 (BIK pT33) Antibody | Human | Polyclonal | Rabbit | BCL2 Interacting Killer Phospho-Thr33 (BIK pT33) | Unconjugated | ELISA, WB, IHC | 412.5 | 100 µl | Q13323 | RUO | |
Abbexa | BIK | abx031835 | Bik (pT33) Antibody | Human | Polyclonal | Rabbit | Bik (pT33) | Unconjugated | ELISA, WB, IHC | 281.25 | 80 µl | Q13323 | RUO |
Te recomendamos que si no encuentras lo que buscas, utilices el buscador, refinando la búsqueda según tu criterio y usando Alias, o bien contacta con nosotros.