WT1 Associated Protein (WTAP) Cell ELISA Kit
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Description
WTAP Cell ELISA Kit is a cell-based ELISA Kit. Cells to be assayed should be seeded onto a clear flat bottom 96 well plate, using poly-L-lysine for non-adherent cells. Cells should be grown to 75-90% confluence and treated prior to carrying out the ELISA.
Documents del producto
Product specifications
| Category | ELISA Kits |
| Immunogen Target | WT1 Associated Protein (WTAP) |
| Reactivity | Human, Mouse |
| Detection Method | Colorimetric |
| Recommended Dilution | Optimal dilutions/concentrations should be determined by the end user. |
| Size 1 | 96 tests |
| Tested Applications | ELISA |
| Availability | Shipped within 1-2 weeks. The validity for this kit is at least 6 months |
| Storage | Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual. |
| Dry Ice | No |
| UniProt ID | Q15007 |
| Gene ID | 9589 |
| OMIM | 605442 |
| Background | Elisa kits for WTAP |
| Status | RUO |
| Note | Validity: 6 months. This product is for research use only. The range and sensitivity is subject to change. Please contact us for the latest product information. For accurate results, sample concentrations must be diluted to mid-range of the kit. If you require a specific range, please contact us in advance or write your request in your order comments. Please note that our ELISA and CLIA kits are optimised for detection of native samples, rather than recombinant proteins. We are unable to guarantee detection of recombinant proteins, as they may have different sequences or tertiary structures to the native protein. |
Descripción
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WTAP antibody
Regulatory subunit of the WMM N6-methyltransferase complex, a multiprotein complex that mediates N6-methyladenosine(m6A) methylation of some adenosine residues of some mRNAs and plays a role in the efficiency of mRNA splicing, processing and mRNA stability. Required for accumulation of METTL3 and METTL14 to nuclear speckle(PubMed:24316715, PubMed:24407421, PubMed:24981863). Acts as a mRNA splicing regulator(PubMed:12444081). Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability(PubMed:17088532). Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes(PubMed:17095724).
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Regulatory subunit of the WMM N6-methyltransferase complex, a multiprotein complex that mediates N6-methyladenosine(m6A) methylation of some adenosine residues of some mRNAs and plays a role in the efficiency of mRNA splicing, processing and mRNA stability. Required for accumulation of METTL3 and METTL14 to nuclear speckle(PubMed:24316715, PubMed:24407421, PubMed:24981863). Acts as a mRNA splicing regulator(PubMed:12444081). Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability(PubMed:17088532). Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes(PubMed:17095724).
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