Signal Transducer And Activator of Transcription 1 Phospho-Ser727 (STAT1 pS727) Antibody
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Description
In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT1 can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens.
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Product specifications
| Category | Primary Antibodies |
| Immunogen Target | Target: Signal Transducer And Activator of Transcription 1 Phospho-Ser727 (STAT1 pS727) Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S727 of human STAT1. |
| Host | Rabbit |
| Reactivity | Human |
| Recommended Dilution | WB: 1/1000, IHC-P: 1/50 - 1/100. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user. |
| Clonality | Polyclonal |
| Conjugation | Unconjugated |
| Isotype | IgG |
| Observed MW | Calculated MW: 87.3 kDa |
| Purification | Purified by protein G affinity chromatography. Then, the antibody fraction was peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody was eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
| Size 1 | 80 µl |
| Size 2 | 400 µl |
| Form | Liquid |
| Tested Applications | ELISA, WB, IHC |
| Buffer | PBS containing 0.09% sodium azide. |
| Availability | Shipped within 5-10 working days. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| Dry Ice | No |
| UniProt ID | P42224 |
| Background | Antibody anti-STAT1 |
| Status | RUO |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |
Descripción
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STAT1 antibody
Signal transducer and transcription activator that mediates cellular responses to interferons(IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN(IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases(TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element(ISRE) to activate the transcription of IFN-stimulated genes(ISG), which drive the cell in an antiviral state. In response to type II IFN(IFN-gamma), STAT1 is tyrosine-and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor(GAF), migrates into the nucleus and binds to the IFN gamma activated sequence(GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Becomes activated in response to KITLG/SCF and KIT signaling. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4.
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