Recombinant Mouse TNFA

Proteins and Peptides

E.Coli

Mouse

Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/ml by gently pipetting 2-3 times, don't vortex.

17.2 kDa

80-235

Greater than 90% as determined by SDS-PAGE.

50μg

200μg

1mg

Lyophilized powder

Western Blot, ELISA

Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH 7.4.

7 days

The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.

P06804

TNF,DIF,TNF-alpha,TNFA,TNFSF2,Tumour necrosis factor,TNF-α,TNLG1F,Tumor necrosis factor alpha, Cachectin

Protein Tnf

RUO

Activity: Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin. The ED50 for this effect is 20-60 pg/mL.
Endotoxin: < 1 EU/µg as determined by LAL test.
Tag : N-terminal His Tag