Recombinant Human PDGFA

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935106861
info@markelab.com
name
Recombinant Human PDGFA
category
Proteins and Peptides
provider
FineTest
reference
P8189
tested applications
Western Blot, ELISA

Documents del producto

Instrucciones
Data sheet
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Product specifications

Category
Proteins and Peptides
Host
E.Coli
Reactivity
Human
Assay Data
Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/ml by gently pipetting 2-3 times, don't vortex.
Observed MW
16.2 kDa
Expression
87-211
Purity
Greater than 90% as determined by SDS-PAGE.
Size 1
50μg
Size 2
200μg
Size 3
1mg
Form
Lyophilized powder
Tested Applications
Western Blot, ELISA
Buffer
Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH 7.4.
Availability
7 days
Storage
The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.
UniProt ID
P04085
Alias
PDGF A, PDGF A chain, PDGF subunit A, PDGF-1, PDGF1, PDGFA, PDGFA_HUMAN, Platelet derived growth factor alpha chain
Background
Protein PDGFA
Status
RUO
Note
Activity: Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells.The ED50 for this effect is 60-230 ng/mL in a fluorometric assay.

Endotoxin: < 1 EU/µg as determined by LAL test.

Tag : N-terminal His Tag

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