Recombinant Human LDHB
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935106861
info@markelab.com
name
Recombinant Human LDHB
category
Proteins and Peptides
provider
FineTest
reference
P1399
tested applications
Western Blot, ELISA
Documents del producto
Instrucciones
Data sheet
Product specifications
| Category | Proteins and Peptides |
| Host | E.Coli |
| Reactivity | Human |
| Assay Data | Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/ml by gently pipetting 2-3 times, don't vortex. |
| Observed MW | 38.5 kDa |
| Expression | 1-334 |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Size 1 | 50μg |
| Size 2 | 200μg |
| Size 3 | 1mg |
| Form | Lyophilized powder |
| Tested Applications | Western Blot, ELISA |
| Buffer | Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH 7.4. |
| Availability | 7 days |
| Storage | The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle. |
| UniProt ID | P07195 |
| Alias | Epididymis secretory protein Li 281, HEL S 281, L lactate dehydrogenase B chain, L-lactate dehydrogenase B chain, Lactate Dehydrogenase B, Lactate dehydrogenase H chain, LDH B, LDH H, LDH heart subunit, LDH-B, LDH-H, LDHB, LDHB_HUMAN, LDHBD, LDHH, Renal carcinoma antigen NY REN 46 |
| Background | Protein LDHB |
| Status | RUO |
| Note | Tag : N-terminal His Tag |
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This gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on chromosomes X, 5 and 13.
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