Recombinant Human EGF

Proteins and Peptides

E.Coli

Human

Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/ml by gently pipetting 2-3 times, don't vortex.

5.7 kDa

971-1023

Greater than 90% as determined by SDS-PAGE.

50μg

200μg

1mg

Lyophilized powder

Western Blot, ELISA

Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 150 mM NaCl with 5% trehalose, pH 7.4.

7 days

The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.

P01133

Beta urogastrone, beta-urogastrone, EGF, EGF_HUMAN, Epidermal growth factor, HOMG4, Pro epidermal growth factor, URG, Urogastrone

Protein EGF

RUO

Activity: Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.1-0.5 ng/mL.

Endotoxin: < 0.01 EU/µg as determined by LAL test.