Rat Tissue Factor (F3) Protein

Proteins and Peptides

Tissue Factor (F3)

E. coli

Activity: Active
Biological Activity: Tissue Factor (TF) is a cell surface glyprotein present in subendothelial tissue and leukocytes. TF is required for the initiation of thrombin formation from the zymogen prothrombin. It has been reported that Epidermal Growth Factor (EGF) can bind with the TF on the hemangioendotheliocytes, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat TF and recombinant rat EGF. Briefly, TF was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to EGF-coated microplate wells and incubated for 2 h at 37°C. Wells were washed with PBST and incubated for 1 h with anti-TF polyclonal antibody, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody, wells were aspirated and washed 3 times. TMB substrate solution was added and wells were incubated for 15-25 minutes at 37 °C. Finally, 50 μl stop solution was added to the wells and the absorbance was read at 450 nm immediately.
Sequence Fragment: Gly30-Glu252
Tag: N-terminal His tag

Rat

Unconjugated

Calculated MW: 26.9 kDa  Observed MW: 29 kDa

Recombinant

> 95%

50 µg

100 µg

200 µg

500 µg

1 mg

Lyophilized

WB, SDS-PAGE

Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.01% Sarcosyl, 5% Trehalose.

Shipped within 5-7 working days.

Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles.

No

P42533

25584

TF,TFA,CD142,Coagulation factor III,Thromboplastin

Protein F3

RUO

THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Endotoxin Level: < 1.0 EU/µg (LAL method)
Concentration: Prior to lyophilization: 400 µg/ml