Rat T-Cell Surface Glycoprotein CD8 Beta Chain (CD8B) Protein
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935106861
info@markelab.com
Precio
234€ (10 µg)
Protein CD8B
proveedor
Abbexareference
abx065943Tested Applications
WB, SDS-PAGEreactivity
status
RUOclonality
Descripción
Cluster Of Differentiation 8b (CD8b) is a recombinant Rat protein produced in a Prokaryotic expression system (E. coli).
Background
The CD8B gene encodes the beta subunit of the CD8 glycoprotein, which typically pairs with CD8A to form the CD8αβ heterodimer. This structure is primarily expressed on cytotoxic T lymphocytes (CTLs), enabling T-cell recognition of MHC class I-peptide complexes. CD8B plays a key role in enhancing TCR signaling and antigen-specific responses by associating with the intracellular signaling protein Lck (lymphocyte-specific protein tyrosine kinase). Unlike CD8A, CD8B is required for high-affinity TCR interactions, making it critical for T-cell activation and target cell lysis. CD8B expression is tightly regulated during T-cell development in the thymus. Dysregulation of CD8B may lead to impaired immune responses, contributing to susceptibility to infections and autoimmune disorders.
Características del producto
category
Proteins and Peptides
origin
Rat
host
E. coli
immunogen target
T-Cell Surface Glycoprotein CD8 Beta Chain (CD8B)
host
E. coli
conjugation
Unconjugated
form
Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
tested applications
WB, SDS-PAGE
size 1
10 µg
size 2
50 µg
size 3
100 µg
size 4
200 µg
size 5
500 µg
observerd MW
Molecular Weight: Calculated MW: 22.6 kDa Observed MW (SDS-PAGE): 23 kDa Concentration: Prior to lyophilization: 200 µg/ml Sequence Fragment: Ala21-Lys208 Tag: N-terminal His tag
expression
Recombinant
purity
> 90%
buffer
Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01% Sarcosyl, 5% Trehalose and Proclin-300.
storage
Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
or code
CD8B
dry ice
No
availability
Shipped within 5-7 working days.
alias
LY3,P37,LEU2,LYT3,Ly-3,CD8B1,CD8beta,T-cell surface glycoprotein CD8 beta chain
uniprot id
note
This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.
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