Rat Protein Wnt-5a (WNT5A) Protein

Proteins and Peptides

Protein Wnt-5a (WNT5A)

Activity: Active
Biological Activity: Protein Wnt-5a (WNT5A) is a ligand for members of the frizzled family of seven transmembrane receptors. It can activate or inhibit canonical Wnt signaling, depending on receptor context. It stimulates cell migration, decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells, and may act as a tumor suppressor. WNT Inhibitory Factor 1 (WIF1) has been identified as an interactor of WNT5A, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat WNT5A and recombinant rat WIF1. Briefly, WNT5A was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 µl were then transferred to WIF1-coated microplate wells and incubated for 2 h at 37°C. Wells were washed with PBST and incubated for 1 h with anti-WNT5A polyclonal antibody, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody, wells were aspirated and washed 3 times. TMB substrate solution was added and wells were incubated for 15-25 minutes at 37 °C. Finally, 50 µl stop solution was added to the wells and the absorbance was read at 450 nm immediately.
Sequence Fragment: Ile62-Lys380
Tag: N-terminal His tag

Rat

Unconjugated

Calculated MW: 39.5 kDa  Observed MW (SDS-PAGE): 40 kDa

Recombinant

> 95%

50 µg

100 µg

200 µg

500 µg

1 mg

Lyophilized

WB, SDS-PAGE

Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.01% Sarcosyl, 5% Trehalose.

Shipped within 5-7 working days.

Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles.

No

Protein WNT5A

RUO

THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Endotoxin Level: < 1.0 EU per 1 µg (LAL method)
Concentration: Prior to lyophilization: 200 µg/ml