Rat Parathyroid Hormone (PTH) Protein

Proteins and Peptides

Parathyroid Hormone (PTH)

E. coli

Activity: Active
Biological Activity: Parathyroid Hormone (PTH) is a hormone secreted by the parathyroid glands, and is important in bone remodeling. Osteoblast-like cell lines that express Parathyroid Hormone 1 Receptor (PTHR1), such as ROS 17/2.8, UMR106, SaOS, U2OS, and MG63, show increased proliferation when exposed to PTH. Rat PTH shares similarities with human PTH; the homology based on amino acid sequence is 71.3%. Therefore, a proliferation assay of recombinant Rat PTH was conducted using U2OS cells. Briefly, U2OS cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of PTH. After incubation for 48 h, cells were observed using an inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, which was incubated for 1-4 hours at 37°C. The absorbance was then measured at 450 nm using a microplate reader. Cell proliferation of U2OS cells after incubation with PTH for 48 h observed by inverted microscope is shown in Figure 3 and Figure 4. Cell viability assessed by CCK-8 is shown in Figure 5, where it can be seen that Rat PTH increased cell viability of U2OS cells.
Sequence Fragment: Ala32-Gln115
Tag: N-terminal His tag and MBP tag

Rat

Unconjugated

Calculated MW: 59.4 kDa  Observed MW (SDS-PAGE): 59 kDa

Recombinant

> 92%

50 µg

100 µg

200 µg

500 µg

1 mg

Lyophilized

WB, SDS-PAGE

Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01% Sarcosyl, 5% Trehalose and Proclin-300.

Shipped within 5-7 working days.

Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles.

No

P04089

Protein PTH

RUO

THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Concentration: Prior to lyophilization: 200 µg/ml