Rat Interleukin 27 (IL27) Protein

category

Proteins and Peptides

origin

Rat

host

293F cell

immunogen target

Interleukin 27 (IL27)

host

293F cell

conjugation

Unconjugated

form

Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.

tested applications

WB, SDS-PAGE

size 1

50 µg

size 2

100 µg

size 3

200 µg

size 4

500 µg

size 5

1 mg

observerd MW

Molecular Weight: Calculated MW: 49.6 kDa Observed MW (SDS-PAGE): 60 kDa Possible reasons why the actual band size differs from the predicted band size: 1. Splice variants. Alternative splicing may create different sized proteins from the same gene. 2. Relative charge. The composition of amino acids may affect the charge of the protein. 3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size. 4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form. 5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed. Concentration: Prior to lyophilization: 50 µg/ml Sequence Fragment: Asp32-Pro234 + (GGGGS)*3 + Gly18-Pro228 Tag: N-terminal His tag

expression

Recombinant

purity

> 90%

buffer

Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01% Sarcosyl, 5% Trehalose and Proclin-300.

storage

Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.

or code

IL27

dry ice

No

availability

Shipped within 5-7 working days.

alias

p28,IL30,IL-27,IL27A,IL-27A,IL27p28,IL-27 subunit alpha,IL-27-A,Interleukin-30,p28

uniprot id

note

This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.