Rat Endonuclease G, Mitochondrial (ENDOG) Protein

Proteins and Peptides

Endonuclease G, Mitochondrial (ENDOG)

E. coli

Activity: Active
Biological Activity: Endonuclease G, Mitochondrial (ENDOG) is a conserved nuclease encoded by nuclear DNA and localized in the mitochondrial intermembrane space. It plays dual role in the apoptosis and mitochondrial genome maintenance. During programmed cell death, ENDOG is released into the cytosol, cleaving nuclear and mitochondrial DNA at specific sites to facilitate apoptotic degradation. Structurally, it functions as a homodimer with Mg2+ dependent endonuclease activity. Beyond apoptosis, ENDOG participates in mitochondrial DNA replication, repair, and maternal genome processing during embryogenesis. Notably, ENDOG interacts with cytochrome c (CYCS) to synergistically amplify apoptosis. This complex enhances nucleolytic DNA degradation and modulates the mitochondrial permeability transition pore (MPTP), further promoting cell death, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat ENDOG and recombinant human CYCS. Briefly, ENDOG was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CYCS-coated microplate wells and incubated for 1 h at 37 °C. Wells were washed with PBST and incubated for 1 h with anti-ENDOG polyclonal antibody, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody, wells were aspirated and washed 5 times. TMB substrate solution was added and wells were incubated 15-25 minutes at 37 °C . Finally, 50 μl stop solution was added to the wells and the absorbance was read at 450 nm immediately.
Sequence Fragment: Met1-Lys294
Tag: N-terminal His tag and GST tag

Rat

Unconjugated

Calculated MW: 62.3 kDa  Observed MW: 62 kDa

Recombinant

> 90%

50 µg

100 µg

200 µg

500 µg

1 mg

Lyophilized

WB, SDS-PAGE

Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl and 5% Trehalose.

Shipped within 5-7 working days.

Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles.

No

Q3V5X8

Protein ENDOG

RUO

THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Endotoxin Level: < 1.0 EU per 1 µg (LAL method)
Concentration: Prior to lyophilization: 350 µg/ml