Pig Fibronectin (FN1) Protein

Proteins and Peptides

Fibronectin (FN1)

E. coli

Activity: Active
Biological Activity: Fibronectin (FN), a large, modular glycoprotein encoded by the FN1 gene, is a key component of the extracellular matrix (ECM) and plasma. Synthesized primarily by fibroblasts, hepatocytes, and endothelial cells, it exists in two major forms: soluble plasma FN and insoluble cellular FN. FN mediates critical cellular processes via its integrin-binding RGD motif, including cell adhesion, migration, proliferation, and differentiation. It also participates in ECM assembly, wound healing, and angiogenesis by bridging cells to other ECM components like collagen, fibrin, and proteoglycans. Dysregulated FN expression or function is linked to pathological conditions such as fibrosis, cancer metastasis, and cardiovascular diseases, making it a vital target for biomedical research. FN binds THBS1, a secreted glycoprotein, to modulate ECM stability and regulate cellular adhesion signaling pathways. Briefly, THBS1 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to FN-coated microtiter wells and incubated for 1 h at 37°C. Wells were washed with PBST and incubated for 1 h with anti-THBS1 polyclonal antibody, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody for 1 h at 37°C, wells were aspirated and washed 5 times. TMB substrate solution was added and wells were incubated 15-25 minutes at 37°C. Finally, add 50 μl stop solution was added to the wells and absorbance was read at 450 nm immediately. Measured by its binding ability in a functional ELISA. When Recombinant FN is immobilized at 2 μg/ml (100 ul/well), the concentration of THBS1 that produces 50% optimal binding response is found to be approximately 0.054 μg/ml.
Sequence Fragment: Pro49-Asp403
Tag: N-terminal His tag

Pig

Unconjugated

Calculated MW: 43.2 kDa  Observed MW (SDS-PAGE): 33 kDa, 45 kDa  Possible reasons why the actual band size differs from the predicted band size: 1. Splice variants. Alternative splicing may create different sized proteins from the same gene. 2. Relative charge. The composition of amino acids may affect the charge of the protein. 3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size. 4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form. 5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed.

Recombinant

> 80%

50 µg

100 µg

200 µg

500 µg

1 mg

Lyophilized

WB, SDS-PAGE

Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl and 5% Trehalose.

Shipped within 5-7 working days.

Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles.

No

F1SS24

Protein FN1

RUO

THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Endotoxin Level: < 1.0 EU per 1 µg (determined by the LAL method)
Concentration: Prior to lyophilization: 200 µg/ml