Pig Brain Derived Neurotrophic Factor (BDNF) Protein

Proteins and Peptides

Brain Derived Neurotrophic Factor (BDNF)

E. coli

Pig

Unconjugated

Molecular Weight: Calculated MW: 27.4 kDa  Observed MW (SDS-PAGE): 32 kDa  Possible reasons why the actual band size differs from the predicted band size: 1. Splice variants. Alternative splicing may create different sized proteins from the same gene. 2. Relative charge. The composition of amino acids may affect the charge of the protein. 3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size. 4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form. 5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed.

Concentration: Prior to lyophilization: 200 µg/ml

Sequence Fragment: Pro20-Arg252

Tag: N-terminal His tag

Recombinant

> 95%

50 µg

100 µg

200 µg

500 µg

1 mg

Lyophilized Reconstitute in ddH2O to a concentration of 0.1-0.2 mg/ml. Do not vortex.

WB, SDS-PAGE

Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01% Sarcosyl, 5% Trehalose.

Shipped within 5-7 working days.

Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.

No

P14082

397495

Protein BDNF

RUO

This product is for research use only.   Not for human consumption, cosmetic, therapeutic or diagnostic use.