Pig Alpha-1-Acid Glycoprotein (a1AGP) Protein
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935106861
info@markelab.com
Precio
429€ (50 µg)
Protein ORM1
proveedor
Abbexareference
abx167520Tested Applications
WB, SDS-PAGEreactivity
status
RUOclonality
Descripción
Pig Alpha-1-Acid Glycoprotein (a1AGP) is a recombinant Pig protein expressed in E. coli.
Background
Alpha-1-Acid Glycoprotein (ORM1), also known as orosomucoid, is an acute-phase plasma protein predominantly synthesized in the liver. ORM1 is a part of the acute-phase response, where its levels in the blood increase in response to inflammation, infection, or injury.It is a 43-44 kDa glycoprotein well known as a one of the major acute phase proteins in humans, rats and many other spices The carbohydrate content represents 45% of the molecular weight attached in the form of five to six highly sialylated complex-type-N-linked glycans.ORM1 has since been studied extensively for its role in immune regulation, drug binding, and inflammation.
Características del producto
category
Proteins and Peptides
origin
Pig
host
E. coli
immunogen target
Alpha-1-Acid Glycoprotein (a1AGP)
host
E. coli
conjugation
Unconjugated
form
Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
tested applications
WB, SDS-PAGE
size 1
50 µg
size 2
100 µg
size 3
200 µg
size 4
500 µg
size 5
1 mg
observerd MW
Molecular Weight: Calculated MW: 23.0 kDa Observed MW (SDS-PAGE): 25 kDa, 27 kDa Possible reasons why the actual band size differs from the predicted band size: 1. Splice variants. Alternative splicing may create different sized proteins from the same gene. 2. Relative charge. The composition of amino acids may affect the charge of the protein. 3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size. 4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form. 5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed. Concentration: Prior to lyophilization: 200 µg/ml Sequence Fragment: Leu2-Glu171 Tag: N-terminal His tag
expression
Recombinant
purity
> 98%
buffer
Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 1 mM DTT, 5% Trehalose and Proclin-300.
storage
Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
or code
ORM1
dry ice
No
availability
Shipped within 5-7 working days.
alias
Alpha-1-Acid Glycoprotein/orm1,Orosomucoid-1,OMD 1,Orosomucoid 1
note
This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.
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