Mouse Tumor Necrosis Factor (TNF) Protein

Este producto es parte de TNF-Tumor Necrosis Factor (Alpha)
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572€ (50 µg)

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935106861
info@markelab.com
name
Mouse Tumor Necrosis Factor (TNF) Protein
category
Proteins and Peptides
provider
Abbexa
reference
abx651569
tested applications
WB, SDS-PAGE

Description

Tumor Necrosis Factor (TNF) Protein is a Mouse protein produced in 293F cell.

Documents del producto

Instrucciones
Data sheet
Descargar

Product specifications

Category
Proteins and Peptides
Immunogen Target
Tumor Necrosis Factor (TNF)
Host
293F cell
Origin
Mouse
Conjugation
Unconjugated
Observed MW
Molecular Weight: Calculated MW: 21.4 kDa  Observed MW: 24 kDa
Concentration: Prior to lyophilization: 200 µg/ml
Sequence Fragment: Gly57-Leu235
Tag: N-terminal His tag
Expression
Recombinant
Purity
> 90%
Size 1
50 µg
Size 2
100 µg
Size 3
200 µg
Size 4
500 µg
Size 5
1 mg
Form
Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Tested Applications
WB, SDS-PAGE
Buffer
Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 5% Trehalose and Proclin-300.
Availability
Shipped within 5-7 working days.
Storage
Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
Dry Ice
No
UniProt ID
P06804
Gene ID
21926
Alias
TNF,DIF,TNF-alpha,TNFA,TNFSF2,Tumour necrosis factor,TNF-α,TNLG1F,Tumor necrosis factor alpha, Cachectin
Background
Protein TNF
Status
RUO
Note
This product is for research use only.   Not for human consumption, cosmetic, therapeutic or diagnostic use.

Descripción

Tumor Necrosis Factor (TNF) is a versatile cytokine involvedl in numerous physiological and pathological processes. It holds a critical role in modulating immune responses, inflammation, and apoptosis. While predominantly produced by activated macrophages, TNF can also originate from various cell types like lymphocytes, mast cells, endothelial cells, and fibroblasts. Two well-known forms of TNF are TNF-alpha and TNF-beta, also referred to as lymphotoxin-alpha. TNF-alpha, being extensively studied, exists in both soluble and membrane-bound forms, both capable of initiating biological responses by binding to their respective receptors. TNF signaling operates through two receptors: TNFR1 and TNFR2. TNFR1 is ubiquitously expressed across cell types, whereas TNFR2 is primarily found in endothelial, epithelial, and certain immune cells. TNFR1 signaling typically triggers pro-inflammatory and apoptotic responses, while TNFR2 signaling exhibits anti-inflammatory properties and fosters cell proliferation. In autoimmune disease treatment, suppressing TNFR1 signaling has proved significant, whereas TNFR2 signaling supports wound healing.The primary function of TNF lies in regulating immune cells

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