Mouse 14-3-3 Protein Sigma (SFN) Protein

Este producto es parte de SFN - stratifin
Mouse 14-3-3 Protein Sigma (SFN) Protein

Pida mas información

935106861

info@markelab.com

Precio

221€ (10 µg)

Protein SFN

proveedor

Abbexa

reference

abx069176

Tested Applications

WB, SDS-PAGE

reactivity

status

RUO

clonality

Descripción

Recombinant 14-3-3 Protein Sigma (SFN) is a recombinant Mouse protein produced in a Prokaryotic expression system (E. coli).


Background

Stratifin (SFN) , also known as 14-3-3 sigma, is a member of the 14-3-3 protein family that acts as a critical regulator of cell cycle progression, DNA damage response, and cellular stress. It binds to phosphorylated serine/threonine residues on target proteins, facilitating their localization, stability, and activity. Stratifin is specifically involved in G2/M cell cycle checkpoint control by sequestering cyclin-dependent kinase complexes like CDC25C in the cytoplasm, preventing premature mitotic entry under DNA damage conditions. It is highly expressed in epithelial tissues and is essential for maintaining epithelial cell integrity, polarity, and stress responses. Stratifin acts as a tumor suppressor by inhibiting uncontrolled proliferation; its downregulation has been observed in breast, lung, and gastrointestinal cancers due to epigenetic silencing. Functional studies reveal its role in mediating stress-induced growth arrest and apoptosis, underscoring its importance in tumor suppression, DNA repair, and cell cycle regulation.

Características del producto

category

Proteins and Peptides


origin

Mouse


host

E. coli


immunogen target

14-3-3 Protein Sigma (SFN)


host

E. coli


conjugation

Unconjugated


form

Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.


tested applications

WB, SDS-PAGE


size 1

10 µg


size 2

50 µg


size 3

100 µg


size 4

200 µg


size 5

500 µg


observerd MW

Molecular Weight: Calculated MW: 31.5 kDa Concentration: Prior to lyophilization: 200 µg/ml Sequence Fragment: Met1-Ser249 Tag: N-terminal His tag


expression

Recombinant


purity

> 95%


buffer

Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 1 mM DTT, 5% Trehalose and Proclin-300.


storage

Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.


or code

SFN


dry ice

No


availability

Shipped within 5-7 working days.


alias

SFN, YWHAS, Stratifin,14-3-3σ protein


note

This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.


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