Mouse 14-3-3 Protein Sigma (SFN) Protein
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935106861
info@markelab.com
Precio
221€ (10 µg)
Protein SFN
proveedor
Abbexareference
abx069176Tested Applications
WB, SDS-PAGEreactivity
status
RUOclonality
Descripción
Recombinant 14-3-3 Protein Sigma (SFN) is a recombinant Mouse protein produced in a Prokaryotic expression system (E. coli).
Background
Stratifin (SFN) , also known as 14-3-3 sigma, is a member of the 14-3-3 protein family that acts as a critical regulator of cell cycle progression, DNA damage response, and cellular stress. It binds to phosphorylated serine/threonine residues on target proteins, facilitating their localization, stability, and activity. Stratifin is specifically involved in G2/M cell cycle checkpoint control by sequestering cyclin-dependent kinase complexes like CDC25C in the cytoplasm, preventing premature mitotic entry under DNA damage conditions. It is highly expressed in epithelial tissues and is essential for maintaining epithelial cell integrity, polarity, and stress responses. Stratifin acts as a tumor suppressor by inhibiting uncontrolled proliferation; its downregulation has been observed in breast, lung, and gastrointestinal cancers due to epigenetic silencing. Functional studies reveal its role in mediating stress-induced growth arrest and apoptosis, underscoring its importance in tumor suppression, DNA repair, and cell cycle regulation.
Características del producto
category
Proteins and Peptides
origin
Mouse
host
E. coli
immunogen target
14-3-3 Protein Sigma (SFN)
host
E. coli
conjugation
Unconjugated
form
Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
tested applications
WB, SDS-PAGE
size 1
10 µg
size 2
50 µg
size 3
100 µg
size 4
200 µg
size 5
500 µg
observerd MW
Molecular Weight: Calculated MW: 31.5 kDa Concentration: Prior to lyophilization: 200 µg/ml Sequence Fragment: Met1-Ser249 Tag: N-terminal His tag
expression
Recombinant
purity
> 95%
buffer
Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 1 mM DTT, 5% Trehalose and Proclin-300.
storage
Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
or code
SFN
dry ice
No
availability
Shipped within 5-7 working days.
alias
SFN, YWHAS, Stratifin,14-3-3σ protein
uniprot id
note
This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.
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