Mouse Protein Wnt-10a (WNT10A) Protein (Active)

Proteins and Peptides

Protein Wnt-10a (WNT10A)

E. coli

Activity: Active
Biological Activity: Wingless Type MMTV Integration Site Family, Member 10A (WNT10A) is a ligand for members of the frizzled family of seven transmembrane receptors. It is involved in the regulation of cell proliferation, differentiation, migration, and apoptosis. Overexpression of WNT10A has been associated with various diseases, including cancer, where it can contribute to tumorigenesis through activation of the Wnt-beta-catenin-TCF signaling pathway. LRP5 and LRP6, as co-receptors of Wnt signal, play a key role in the signal transduction process, therefore a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse WNT10A and recombinant rat LRP5. Briefly, WNT10A was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to LRP5-coated microplate wells and incubated for 1 h at 37°C. Wells were washed with PBST and incubated for 1 h with anti-WNT10A polyclonal antibody, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody for 1 h at 37°C, wells were aspirated and washed 5 times. TMB substrate solution was added and wells were incubated for 15-25 minutes at 37 °C. Finally, 50 μl stop solution was added to the wells and the absorbance was read at 450/630 nm immediately.
Sequence Fragment: Ser108-Pro343
Tag: N-terminal His tag and GST tag

Mouse

Unconjugated

Calculated MW: 56.0 kDa  Observed MW (SDS-PAGE): 56 kDa

Recombinant

> 80%

50 µg

100 µg

200 µg

500 µg

1 mg

Lyophilized

WB, SDS-PAGE

Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 5% Trehalose.

Shipped within 5-7 working days.

Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles.

No

P70701

22409

Protein WNT10A

RUO

THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/ml. Do not vortex.
Endotoxin Level: < 1.0 EU per 1 µg (LAL method)
Concentration: Prior to lyophilization: 50 µg/ml