Mouse Neutrophil Elastase (ELANE) Protein

Proteins and Peptides

Neutrophil Elastase (ELANE)

293F cells

Activity: Active
Biological Activity: A functional binding ELISA assay was conducted to detect the interaction of recombinant Mouse Neutrophil Elastase (NE) and recombinant Goat Lactoferrin (LTF). Biotin-conjugated NE was serially diluted in PBS, with 0.01% BSA, pH 7.4. 100 μl duplicates were then transferred to LTF-coated microtiter wells and incubated for 1h at 37°C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30 mins. The wells were then aspirated and washed 5 times. Substrate solution was added and the wells were incubated for 15-25 minutes at 37°C. 50 μl stop solution was added to the wells and read at 450 nm. The EC50 for this effect was determined to be 0.084 µg/ml.
Sequence Fragment: Ser27-Asn265
Tag: N-terminal His Tag

Mouse

Unconjugated

Calculated MW: 21.7 kDa  Observed MW (SDS-PAGE): 33 kDa  Possible reasons why the actual band size differs from the predicted band size: 1. Splice variants. Alternative splicing may create different sized proteins from the same gene. 2. Relative charge. The composition of amino acids may affect the charge of the protein. 3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size. 4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form. 5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed.

Recombinant

> 97%

50 µg

100 µg

200 µg

500 µg

1 mg

Lyophilized

WB, SDS-PAGE

Prior to lyophilization: PBS, pH 7.4, containing 5% Trehalose.

Shipped within 5-7 working days.

Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles.

No

Q3UP87

50701

Protein ELANE

RUO

THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Concentration: Prior to lyophilization: 500 µg/ml