Mouse Activating molecule in BECN1-regulated autophagy protein 1 (AMBRA1) ELISA Kit

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ELISA Kits

Activating molecule in BECN1-regulated autophagy protein 1 (AMBRA1)

Mouse

Colorimetric

Quantitative

0.156 ng/ml - 10 ng/ml

Optimal dilutions/concentrations should be determined by the end user.

96 tests

Standard Form: Lyophilized

ELISA

Tissue homogenates, cell lysates and other biological fluids.

Shipped within 5-15 working days.

Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.

No

A2AH22

228361

AMBRA1,DCAF3,DDB1 and CUL4-associated factor 3

Elisa Kits for: AMBRA1

RUO

THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES. The range and sensitivity is subject to change. Please contact us for the latest product information. For accurate results, sample concentrations must be diluted to mid-range of the kit. If you require a specific range, please contact us in advance or write your request in your order comments. Please note that our kits are optimised for detection of native samples, rather than recombinant proteins. We are unable to guarantee detection of recombinant proteins, as they may have different sequences or tertiary structures to the native protein.
The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5% within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.

Background

AMBRA1 or Activating Molecule in BECN1-regulated Autophagy Protein 1, is a key regulator of the autophagy process in mammalian cells. Autophagy is a cellular process involved in the degradation and recycling of damaged organelles, misfolded proteins, and other cellular components to maintain cellular homeostasis and respond to stress conditions. AMBRA1 was originally identified as a positive regulator of autophagy that interacts with Beclin 1 (encoded by the BECN1 gene), a core component of the class III phosphatidylinositol 3-kinase (PI3K) complex involved in autophagosome formation. Through its interactions with Beclin 1 and other autophagy-related proteins, AMBRA1 plays a crucial role in initiating and regulating the autophagy process.AMBRA1 interacts with Beclin 1 and helps to stabilize the Beclin 1 complex, promoting the nucleation of autophagosomal membranes and the initiation of autophagy. AMBRA1 regulates the size and maturation of autophagosomes, the double-membrane vesicles that sequester cargo for degradation during autophagy. It interacts with various proteins involved in autophagosome formation and fusion with lysosomes. AMBRA1 is also involved in crosstalk with signaling pathways that regulate autophagy, such as the mammalian target of rapamycin (mTOR) pathway and the p53 pathway. It can be phosphorylated and regulated by these pathways to modulate autophagy in response to cellular stress and nutrient availability.

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