Matrix Metalloproteinase 13 (MMP13) Antibody

Pida mas información

935106861

info@markelab.com

Precio

850€ (1 ml)

proveedor

Abbexa

reference

abx173485

Tested Applications

WB, IHC, IF/ICC

reactivity

Pig

status

RUO

clonality

Monoclonal

Descripción

This product is currently in development. The lead time for this product may be several months. Please contact us at info@markelab.com for an updated lead time before purchasing this product. Matrix Metalloproteinase 13 (MMP13) Antibody is a Mouse Monoclonal antibody against Matrix Metalloproteinase 13 (MMP13).

Background

MMPs are secreted by diverse connective tissue and pro-inflammatory cells. Typically, these enzymes exhibit minimal expression under normal physiological circumstances. MMPs assume a pivotal role in various cellular processes, including cell proliferation, migration, differentiation, angiogenesis, apoptosis, and immunity. They share a common domain structure, consisting of three key components. First is the pro-peptide, which requires removal to activate the enzyme. Next, there is the catalytic domain, featuring a cysteine switch, where a cysteine residue interacts with zinc, and the haemopexin-like C-terminal domain is linked to the catalytic domain via a flexible hinge region. Collagenase 3, also known as MMP-13, is an enzyme encoded by the MMP13 gene in humans. It is also recognized by its aliases CLG3, MANDP1, and MDST. MMP-13 is particularly efficient at cleaving type II collagen, more so than types I and III. It is thought to be involved in the turnover of articular cartilage and is associated with the pathophysiology of osteoarthritis. The activity of MMP-13 is tightly regulated due to its potent proteolytic capabilities. Various cytokines and growth factors have been shown to influence the expression of the Mmp13 gene, including parathyroid hormone, IGF-1, TGF-β, hepatocyte growth factor, as well as several inflammatory cytokines such as IL-1α and IL-1β.

Background

MMPs son secretadas por diversos tejidos conectivos y células proinflamatorias. Normalmente, estas enzimas exhiben una expresión mínima en circunstancias fisiológicas normales. Las MMP asumen un papel fundamental en diversos procesos celulares, incluida la proliferación, migración, diferenciación, angiogénesis, apoptosis e inmunidad celular. Comparten una estructura de dominio común, que consta de tres componentes clave. El primero es el propéptido, que requiere eliminación para activar la enzima. A continuación, está el dominio catalítico, que presenta un interruptor de cisteína, donde un residuo de cisteína interactúa con el zinc, y el dominio C-terminal similar a hemopexina está unido al dominio catalítico a través de una región bisagra flexible. La colagenasa 3, también conocida como MMP-13, es una enzima codificada por el gen MMP13 en humanos. También se le reconoce por sus alias CLG3, MANDP1 y MDST. MMP-13 es particularmente eficaz para escindir el colágeno tipo II, más que los tipos I y III. Se cree que participa en la renovación del cartílago articular y está asociado con la fisiopatología de la osteoartritis. La actividad de MMP-13 está estrictamente regulada debido a sus potentes capacidades proteolíticas. Se ha demostrado que varias citocinas y factores de crecimiento influyen en la expresión del gen Mmp13, incluida la hormona paratiroidea, IGF-1, TGF-β, factor de crecimiento de hepatocitos, así como varias citocinas inflamatorias como IL-1α e IL-1β.

Características del producto

clonality

Monoclonal

reactivity

Pig

immunogen target

Matrix Metalloproteinase 13 (MMP13)

host

Mouse

conjugation

Unconjugated

form

Liquid

tested applications

WB, IHC, IF/ICC

purification

Purified by Protein A and Protein G affinity chromatography.

buffer

0.01 M PBS, pH 7.4, containing 0.05% Proclin-300, 50% glycerol.

size 1

1 ml

storage

Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.

or code

MMP13

dry ice

availability

Please enquire.

note

This product is for research use only.

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