Características de producto
2 – 8 °C
0/ 100 – 10 000 ng/ml
Common sample preparation. Derivatization in liquid phase (96-well-reaction plate). Immunoassay using 12 x 8 break apart wells microtiter plate (precoated). 6 standards. 2 controls.
Sample preparation and acylation 1 hour 30 min; ELISA overnight, 2 x 30 min
10 µl serum or plasma
Enzyme Immunoassay for the quantitative determination of L-Kynurenine in serum and EDTA plasma samples for the determination of kynurenine homeostasis. After acylation Kynurenine is quantitatively determined by ELISA. The competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples and the solid phase bound analyte compete for a fixed number of antibody binding sites. When the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm