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Kynurenine ELISA

Enzyme immunoassay (ELISA) for the quantitative determination of kynurenine in serum and plasma.

Proveedor

LDN

Reference

BAE2200

Tested Applications

Elisa

Reactivity

Human

Status

CE Marked

Características de producto

tested_applications
Elisa
reactivity
Human
category
ELISA Kits
size_1
96 test
storage
2 – 8 °C
test_range
0/ 100 – 10 000 ng/ml
sensitivity
45.7 ng/ml
detection_method
Common sample preparation. Derivatization in liquid phase (96-well-reaction plate). Immunoassay using 12 x 8 break apart wells microtiter plate (precoated). 6 standards. 2 controls.
assay_data
Sample preparation and acylation 1 hour 30 min; ELISA overnight, 2 x 30 min
availability
Inmediate
dry_ice
No
note
10 µl serum or plasma
reserach_area
Kynurenine
background
Enzyme Immunoassay for the quantitative determination of L-Kynurenine in serum and EDTA plasma samples for the determination of kynurenine homeostasis. After acylation Kynurenine is quantitatively determined by ELISA. The competitive ELISA uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The acylated standards, controls and samples and the solid phase bound analyte compete for a fixed number of antibody binding sites. When the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm