Importin Subunit Alpha-2 (KPNA2) Antibody
Por favor contáctenos para obtener información detallada sobre el precio y disponibilidad.
Description
KPNA2 Antibody is a Rabbit Polyclonal antibody against KPNA2. The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination [provided by RefSeq, Jul 2008].
Documents del producto
Product specifications
| Category | Primary Antibodies |
| Immunogen Target | Importin Subunit Alpha-2 (KPNA2) |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Recommended Dilution | ELISA: 1 µg/ml, WB: 1/500 - 1/2000, IP: 0.5 µg - 4 µg antibody per 200 µg - 400 µg extracts of whole cells. Optimal dilutions/concentrations should be determined by the end user. |
| Clonality | Polyclonal |
| Conjugation | Unconjugated |
| Isotype | IgG |
| Purification | Purified by affinity chromatography. |
| Size 1 | 20 µl |
| Size 2 | 100 µl |
| Size 3 | 2 × 100 µl |
| Form | Liquid |
| Tested Applications | ELISA, WB, IP |
| Buffer | PBS, pH 7.3, containing 0.02% sodium azide, 50% glycerol. |
| Availability | Shipped within 5-10 working days. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| Dry Ice | No |
| UniProt ID | P52292 |
| Gene ID | 3838 |
| NCBI Accession | NP_002257.1 |
| Alias | KPNA2,IPOA1, QIP2, RCH1, SRP1alpha, Karyopherin alpha 2, SRP1-alpha, karyopherin subunit alpha 2, PTAC58 |
| Background | Antibody anti-KPNA2 |
| Status | RUO |
| Note | Concentration: > 0.2 mg/ml - |
Descripción
KPNA2, also known as importin-α2, is a key nuclear transport adaptor protein that binds cargo proteins containing classical nuclear localization signals (NLS). It works in conjunction with importin-β to translocate the cargo through the nuclear pore complex (NPC) into the nucleus. KPNA2 is ubiquitously expressed and is involved in processes like gene expression, DNA repair, and cell proliferation. It has been shown to play a significant role in cancer biology, where overexpression of KPNA2 is associated with tumor progression, metastasis, and poor prognosis in cancers such as breast, lung, and liver cancers. KPNA2 also facilitates the nuclear import of viral proteins, making it a target in viral pathogenesis. Knockout studies indicate that KPNA2 is essential for cell viability and differentiation, particularly during embryogenesis and immune cell development.
Related Products
Human KPNA2 (Importin subunit alpha-2) ELISA Kit
Ver Producto
KPNA2 antibody
Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex(NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and-beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP-and GDP-bound forms of Ran between the cytoplasm and nucleus.
Ver Producto