Human WT1 Associated Protein (WTAP) Protein

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Description
Human WTAP Protein is a recombinant protein from Human produced in E. coli. A DNA sequence encoding the human WTAP isoform 2 (Q15007-2) (Met 1-Arg 151) was fused with the GST tag at the N-terminus.
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Product specifications
| Category | Proteins and Peptides |
| Immunogen Target | WTAP |
| Host | E. coli |
| Origin | Human |
| Observed MW | Molecular Weight: 45 kDa Sequence Fragment: Met1-Arg151 Tag: N-terminal GST tag Validity: The validity for this protein is 12 months. |
| Expression | Recombinant |
| Purity | > 90% (SDS-PAGE) |
| Size 1 | 100 µg |
| Tested Applications | SDS-PAGE |
| Buffer | Lyophilized from sterile 20mM Tris, 0.15M NaCl, 0.5mM GSH, pH 8.0. |
| Availability | Shipped within 5-15 working days. |
| Storage | Aliquot and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. |
| Dry Ice | No |
| UniProt ID | Q15007-2 |
| Background | Protein WTAP |
| Status | RUO |
| Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |
Descripción
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Regulatory subunit of the WMM N6-methyltransferase complex, a multiprotein complex that mediates N6-methyladenosine(m6A) methylation of some adenosine residues of some mRNAs and plays a role in the efficiency of mRNA splicing, processing and mRNA stability. Required for accumulation of METTL3 and METTL14 to nuclear speckle(PubMed:24316715, PubMed:24407421, PubMed:24981863). Acts as a mRNA splicing regulator(PubMed:12444081). Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability(PubMed:17088532). Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes(PubMed:17095724).
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Regulatory subunit of the WMM N6-methyltransferase complex, a multiprotein complex that mediates N6-methyladenosine(m6A) methylation of some adenosine residues of some mRNAs and plays a role in the efficiency of mRNA splicing, processing and mRNA stability. Required for accumulation of METTL3 and METTL14 to nuclear speckle(PubMed:24316715, PubMed:24407421, PubMed:24981863). Acts as a mRNA splicing regulator(PubMed:12444081). Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability(PubMed:17088532). Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes(PubMed:17095724).
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