Human Neurogenic Locus Notch Homolog Protein 1 (NOTCH1) Protein

Este producto es parte de NOTCH - Neurogenic locus notch homolog protein
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208€ (10 µg)

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935106861
info@markelab.com
name
Human Neurogenic Locus Notch Homolog Protein 1 (NOTCH1) Protein
category
Proteins and Peptides
provider
Abbexa
reference
abx655331
tested applications
WB, SDS-PAGE

Description

Human Neurogenic Locus Notch Homolog Protein 1 (NOTCH1) Protein is a Recombinant Human protein expressed in E. coli.

Documents del producto

Instrucciones
Data sheet
Descargar

Product specifications

Category
Proteins and Peptides
Immunogen Target
Neurogenic Locus Notch Homolog Protein 1 (NOTCH1)
Host
E. coli
Origin
Human
Conjugation
Unconjugated
Observed MW
Molecular Weight: Calculated MW: 31.7 kDa  Observed MW (SDS-PAGE): 36 kDa  Possible reasons why the actual band size differs from the predicted band size: 1. Splice variants. Alternative splicing may create different sized proteins from the same gene. 2. Relative charge. The composition of amino acids may affect the charge of the protein. 3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size. 4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form. 5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed.
Concentration: Prior to lyophilization: 200 µg/ml
Sequence Fragment: Glu1949-Tyr2209
Tag: N-terminal His tag
Expression
Recombinant
Purity
> 90%
Size 1
10 µg
Size 2
50 µg
Size 3
100 µg
Size 4
200 µg
Size 5
500 µg
Form
Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Tested Applications
WB, SDS-PAGE
Buffer
Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 5% Trehalose.
Availability
Shipped within 1-2 months.
Storage
Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
Dry Ice
No
UniProt ID
P46531
Gene ID
4851
Alias
AOS5,AOVD1,TAN1,Hn1,Translocation-associated notch protein TAN-1,notch receptor 1
Background
Protein NOTCH1
Status
RUO
Note
This product is for research use only.   Not for human consumption, cosmetic, therapeutic or diagnostic use.

Descripción

NOTCH1 is a transmembrane receptor of the Notch signaling pathway, which regulates critical processes such as cell fate determination, proliferation, differentiation, and apoptosis during development and tissue homeostasis. Upon ligand binding (e.g., Jagged or Delta-like ligands), the NOTCH1 receptor undergoes proteolytic cleavage, releasing its intracellular domain (NICD). NICD translocates to the nucleus, activating transcription of target genes, including HES and HEY family members, to modulate cell behavior. Dysregulation of NOTCH1 contributes to various diseases, including cancers such as T-cell acute lymphoblastic leukemia (T-ALL), where activating mutations drive aberrant proliferation. In addition, NOTCH1 plays a role in cardiovascular development, neural differentiation, and immune system regulation. Knockout studies reveal embryonic lethality due to disrupted vascular development and neurogenesis, demonstrating its essential role in embryonic and adult tissue homeostasis, cellular differentiation, and oncogenesis.

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