Human 14-3-3 Protein Sigma (SFN) Protein

Este producto es parte de SFN - stratifin
Human 14-3-3 Protein Sigma (SFN) Protein
195€ (10 µg)

Por favor contáctenos para obtener información detallada sobre el precio y disponibilidad.

Name
Human 14-3-3 Protein Sigma (SFN) Protein
Category
Proteins and Peptides
Provider
Abbexa
Reference
abx069174
Tested Applications
WB, SDS-PAGE

Description

Human 14-3-3 Protein Sigma (SFN) is a recombinant Human protein expressed in E. coli.

Documentos del producto

Instrucciones
Data sheet
Descargar

Especificaciones del producto

Category
Proteins and Peptides
Immunogen Target
14-3-3 Protein Sigma (SFN)
Host
E. coli
Assay Type
Activity: Not tested
Sequence Fragment: Met1-Ser248
Tag: N-terminal His tag
Origin
Human
Conjugation
Unconjugated
Observed MW
Calculated MW: 29.3 kDa
Expression
Recombinant
Purity
> 95%
Size 1
10 µg
Size 2
50 µg
Size 3
100 µg
Size 4
200 µg
Size 5
500 µg
Form
Lyophilized
Tested Applications
WB, SDS-PAGE
Buffer
Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 1 mM DTT, 5% Trehalose and Proclin-300.
Availability
Shipped within 5-7 working days.
Storage
Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles.
Dry Ice
No
UniProt ID
P31947
Alias
SFN, YWHAS, Stratifin,14-3-3σ protein
Background
Protein SFN
Status
RUO
Note
THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Concentration: Prior to lyophilization: 200 µg/ml

Background

Stratifin (SFN) , also known as 14-3-3 sigma, is a member of the 14-3-3 protein family that acts as a critical regulator of cell cycle progression, DNA damage response, and cellular stress. It binds to phosphorylated serine/threonine residues on target proteins, facilitating their localization, stability, and activity. Stratifin is specifically involved in G2/M cell cycle checkpoint control by sequestering cyclin-dependent kinase complexes like CDC25C in the cytoplasm, preventing premature mitotic entry under DNA damage conditions. It is highly expressed in epithelial tissues and is essential for maintaining epithelial cell integrity, polarity, and stress responses. Stratifin acts as a tumor suppressor by inhibiting uncontrolled proliferation; its downregulation has been observed in breast, lung, and gastrointestinal cancers due to epigenetic silencing. Functional studies reveal its role in mediating stress-induced growth arrest and apoptosis, underscoring its importance in tumor suppression, DNA repair, and cell cycle regulation.

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