Human Sex Hormone Binding Globulin (SHBG) Protein

Proteins and Peptides

Sex Hormone Binding Globulin (SHBG)

293F cells

Activity: Active
Biological Activity: Sex Hormone-Binding Globulin (SHBG), also known as Sex Steroid-Binding Globulin (SSBG), is a glycoprotein that binds to the sex hormones estrogen, dihydrotesterone (DHT) and testerone. SHBG transports these hormones in blood to tissues in the body. A binding ELISA assay was conducted to detect the interaction of recombinant human SHBG and BSA-conjugated Testosterone. Briefly, biotin-conjugated SHBG was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to BSA-conjugated Testosterone-coated microplate wells and incubated for 1 h at 37°C. Wells were washed with PBST 3 times and incubated for 30 min with HRP-conjugate. Wells were aspirated and washed 5 times. TMB substrate solution was added and wells were incubated for 15-25 minutes at 37 °C. Finally, 50 μl stop solution was added to the wells and the absorbance was read at 450 nm immediately. The binding activity of SHBG and Testosterone is shown in Figure 4.
Sequence Fragment: Arg31-Ser401
Tag: N-terminal His tag

Human

Unconjugated

Calculated MW: 42.0 kDa  Observed MW (SDS-PAGE): 50 kDa

Recombinant

> 99%

50 µg

100 µg

200 µg

500 µg

1 mg

Lyophilized

WB, SDS-PAGE

Prior to lyophilization: 10 mM PBS, pH 7.6, containing 5% Trehalose.

Shipped within 5-7 working days.

Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles.

No

P04278

6462

182205

Protein SHBG

RUO

THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.6. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.6, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Endotoxin Level: < 1.0 EU/µg (LAL method)
Concentration: Prior to lyophilization: 200 µg/ml