Human S100 Calcium Binding Protein A6 (S100A6) Protein

Proteins and Peptides

S100 Calcium Binding Protein A6 (S100A6)

E. coli

Activity: Active
Biological Activity: Human S100 Calcium Binding Protein A6 (S100A6) is a calcium-binding protein that functions as calcium sensor and modulator, contributing to cellular calcium signaling. It is reported that S100B and S100A6 differentially modulate cell survival by interacting with RAGE (Receptor for Advanced Glycation End Products, also known as AGER). Therefore a binding ELISA assay was conducted to detect the interaction of S100A6 and RAGE. Briefly, S100A6 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 µl were then transferred to RAGE-coated microplate wells and incubated for 2 h at 37°C. Wells were washed with PBST and incubated for 1 h with anti-S100A6 monoclonal antibody, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody, wells were aspirated and washed 3 times. TMB substrate solution was added and wells were incubated for 15-25 minutes at 37 °C. Finally, 50 µl stop solution was added to the wells and the absorbance was read at 450 nm immediately.
Sequence Fragment: Met1-Gly90
Tag: N-terminal His tag

Human

Unconjugated

Calculated MW: 16.9 kDa  Observed MW: 16 kDa

Recombinant

> 92%

50 µg

100 µg

200 µg

500 µg

1 mg

Lyophilized

WB, SDS-PAGE

Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.01% Sarcosyl, 5% Trehalose.

Shipped within 5-7 working days.

Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles.

No

P06703

Protein S100A6

RUO

THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Concentration: Prior to lyophilization: 200 µg/ml