Human Pro-Opiomelanocortin (POMC) Protein

category

Proteins and Peptides

origin

Human

host

E. coli

immunogen target

Pro-Opiomelanocortin (POMC)

host

E. coli

conjugation

Unconjugated

form

Lyophilized Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/ml. Do not vortex.

tested applications

WB, SDS-PAGE

size 1

10 µg

size 2

50 µg

size 3

100 µg

size 4

200 µg

size 5

500 µg

observerd MW

Molecular Weight: Calculated MW: 30.9 kDa Observed MW (SDS-PAGE): 37 kDa Possible reasons why the actual band size differs from the predicted band size: 1. Splice variants. Alternative splicing may create different sized proteins from the same gene. 2. Relative charge. The composition of amino acids may affect the charge of the protein. 3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size. 4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form. 5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed. Concentration: Prior to lyophilization: 200 µg/ml Sequence Fragment: Glu23-Glu267 Tag: N-terminal His tag

expression

Recombinant

purity

> 90%

buffer

Prior to lyophilization: 100 mM NaHCO₃, 500 mM NaCl, pH 8.3, containing 1 mM EDTA, 1 mM DTT, 0.01% Sarcosyl, 5% Trehalose and Proclin-300.

storage

Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.

or code

POMC

dry ice

No

availability

Shipped within 5-7 working days.

alias

LPH,MSH,NPP,POC,ACTH,CLIP,OBAIRH,Corticotropin,lipotropin

uniprot id

gene id

note

This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.