Human Prion Protein (PRNP) Protein
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935106861
info@markelab.com
Precio
455€ (50 µg)
Protein PRNP
proveedor
Abbexareference
abx655889Tested Applications
WB, SDS-PAGEreactivity
status
RUOclonality
Descripción
Human Prion Protein (PRNP) is a recombinant Human protein produced in 293F cells using Eukaryotic expression.
Background
The PRNP gene, encoding the prion protein (PrP), plays a critical role in both neurobiology and disease. This gene is located on chromosome 20 in humans and encodes the cellular prion protein, PrP^C. Prion proteins are unique in their ability to exist in multiple conformations, including a pathological form, PrP^Sc, associated with prion diseases. Prion diseases, also known as transmissible spongiform encephalopathies (TSEs), are neurodegenerative disorders marked by rapidly progressive neuronal loss and a spongiform appearance in brain tissue. TSEs include diseases such as Creutzfeldt-Jakob disease (CJD), fatal familial insomnia (FFI), Gerstmann-Sträussler-Scheinker syndrome (GSS), and kuru. The prion protein (PrP) is primarily expressed in the central nervous system (CNS), especially in neurons, but it is also found in other tissues. While the physiological function of PrP^C remains partially understood, it is thought to be involved in cellular processes like cell signaling, protection against oxidative stress, and synaptic function. Its pathological form, PrP^Sc, is formed by a misfolded conformation of PrP^C that becomes self-propagating, causing other normal PrP^C molecules to misfold. This cascade leads to the accumulation of toxic aggregates and extensive neuronal damage, a hallmark of prion diseases.
Características del producto
category
Proteins and Peptides
origin
Human
host
293F cell
immunogen target
Prion Protein (PRNP)
host
293F cell
conjugation
Unconjugated
form
Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
tested applications
WB, SDS-PAGE
size 1
50 µg
size 2
100 µg
size 3
200 µg
size 4
500 µg
size 5
1 mg
observerd MW
Molecular Weight: Calculated MW: 24.3 kDa Observed MW (SDS-PAGE): 25 kDa Concentration: Prior to lyophilization: 150 µg/ml Sequence Fragment: Lys23-Ser230 Tag: N-terminal His tag
expression
Recombinant
purity
> 95%
buffer
Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.01% Sarcosyl, 5% Trehalose.
storage
Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
or code
PRNP
dry ice
No
availability
Shipped within 5-7 working days.
alias
Creutzfeldt-Jakob disease,CJD,GSS,PrP,ASCR,KURU,PRIP,PrPc,CD230,Alternative prion protein
uniprot id
note
This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.
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