Human Microphthalmia Associated Transcription Factor (MITF) Protein

208€ (10 µg)
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935106861
info@markelab.com
name
Human Microphthalmia Associated Transcription Factor (MITF) Protein
category
Proteins and Peptides
provider
Abbexa
reference
abx652247
tested applications
WB, SDS-PAGE
Description
Microphthalmia Associated Transcription Factor (MITF) Protein is a Recombinant protein from Human.
Documents del producto
Instrucciones
Data sheet
Product specifications
Category | Proteins and Peptides |
Immunogen Target | Microphthalmia Associated Transcription Factor (MITF) |
Host | E. coli |
Origin | Human |
Conjugation | Unconjugated |
Observed MW | Molecular Weight: Calculated MW: 61.9 kDa Observed MW (SDS-PAGE): 62 kDa Concentration: Prior to lyophilization: 200 µg/ml Sequence Fragment: RPH645Hu01 Tag: N-terminal His tag |
Expression | Recombinant |
Purity | > 95% |
Size 1 | 10 µg |
Size 2 | 50 µg |
Size 3 | 100 µg |
Size 4 | 200 µg |
Size 5 | 500 µg |
Form | Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex. |
Tested Applications | WB, SDS-PAGE |
Buffer | Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01% Sarcosyl, 5% Trehalose and Proclin-300. |
Availability | Shipped within 5-7 working days. |
Storage | Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles. |
Dry Ice | No |
UniProt ID | O75030 |
Background | Protein MITF |
Status | RUO |
Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |
Descripción
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Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to symmetrical DNA sequences(E-boxes)(5'-CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase(TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase(TYR) and tyrosinase-related protein 1(TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.
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