Human Metalloproteinase Inhibitor 2 (TIMP2) ELISA Kit
proveedor
Abbexareference
abx350299Tested Applications
ELISAreactivity
Humanstatus
RUOclonality
Descripción
Human Metalloproteinase Inhibitor 2 (TIMP2) ELISA Kit is an ELISA Kit for the in vitro quantitative measurement of Human Metalloproteinase Inhibitor 2 (TIMP2) concentrations in serum, plasma and other biological fluids.
Background
MMPs are secreted by diverse connective tissue and pro-inflammatory cells. Typically, these enzymes exhibit minimal expression under normal physiological circumstances. MMPs assume a pivotal role in various cellular processes, including cell proliferation, migration, differentiation, angiogenesis, apoptosis, and immunity. They share a common domain structure, consisting of three key components. First is the pro-peptide, which requires removal to activate the enzyme. Next, there is the catalytic domain, featuring a cysteine switch, where a cysteine residue interacts with zinc, and the haemopexin-like C-terminal domain is linked to the catalytic domain via a flexible hinge region. TIMP-2 (Tissue Inhibitor of Metalloproteinases 2) is indeed a natural inhibitor of the matrix metalloproteinases (MMPs). It forms complexes with certain enzymes within the metalloproteinase family, leading to their irreversible inactivation. TIMP-2 is known to act on a wide range of MMPs, including MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16, and MMP-19. Interestingly, TIMP-2 shares a significant degree of amino acid sequence homology, about 40%, with TIMP-1, especially in their N-terminal domains. This structural similarity underscores their related functions as inhibitors of metalloproteinases, despite some differences in their specific roles and affinities for certain MMPs.
Background
MMPs son secretadas por diversos tejidos conectivos y células proinflamatorias. Normalmente, estas enzimas exhiben una expresión mínima en circunstancias fisiológicas normales. Las MMP asumen un papel fundamental en diversos procesos celulares, incluida la proliferación, migración, diferenciación, angiogénesis, apoptosis e inmunidad celular. Comparten una estructura de dominio común, que consta de tres componentes clave. El primero es el propéptido, que requiere eliminación para activar la enzima. A continuación, está el dominio catalítico, que presenta un interruptor de cisteína, donde un residuo de cisteína interactúa con el zinc, y el dominio C-terminal similar a hemopexina está unido al dominio catalítico a través de una región bisagra flexible. TIMP-2 (inhibidor tisular de metaloproteinasas 2) es de hecho un inhibidor natural de las metaloproteinasas de matriz (MMP). Forma complejos con determinadas enzimas de la familia de las metaloproteinasas, lo que lleva a su inactivación irreversible. Se sabe que TIMP-2 actúa sobre una amplia gama de MMP, incluidas MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14. , MMP-15, MMP-16 y MMP-19. Curiosamente, TIMP-2 comparte un grado significativo de homología de secuencia de aminoácidos, alrededor del 40%, con TIMP-1, especialmente en sus dominios N-terminales. Esta similitud estructural subraya sus funciones relacionadas como inhibidores de metaloproteinasas, a pesar de algunas diferencias en sus funciones específicas y afinidades por ciertas MMP.
Características del producto
category
ELISA Kits
immunogen target
Metalloproteinase Inhibitor 2 (TIMP2)
reactivity
Human
test range
0.156 ng/ml - 10 ng/ml
sensitivity
0.1 ng/ml
assay type
Sandwich
assay data
Quantitative
detection method
Colorimetric
sample type
Serum,Plasma,Other biological fluids
size 1
96 tests
storage
Enviado a 4 °C. Una vez recibido, almacene el kit de acuerdo con las instrucciones citadas en el manual del kit
or code
TIMP2
dry ice
No
uniprot id
note
This product is for research use only. <p></p> The range and sensitivity is subject to change. Please contact us for the latest product information. For accurate results, sample concentrations must be diluted to mid-range of the kit. If you require a specific range, please contact us in advance or write your request in your order comments. <p></p> Please note that our ELISA and CLIA kits are optimised for detection of native samples, rather than recombinant proteins. We are unable to guarantee detection of recombinant proteins, as they may have different sequences or tertiary structures to the native protein.
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