Human Lemur Tyrosine Kinase 3 (LMTK3) Protein

Proteins and Peptides

Lemur Tyrosine Kinase 3 (LMTK3)

E. coli

Activity: Active
Biological Activity: Lemur tyrosine kinase 3 (LMTK3) is implicated in breast cancer growth and endocrine resistance, and has been reported to promote cell invasion, motility, and migration of the MCF-7 breast cancer cell line. A proliferation assay was conducted to detect the bioactivity of recombinant Human LMTK3 using MCF-7 cells. Briefly, MCF-7 cells were seeded into triplicate wells in 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free DMEM prior to the addition of various concentrations of LMTK3. After incubation for 72 h, cells were observed by inverted microscope (Figures 3 and 4) and cell proliferation was measured by Cell Counting Kit-8 (CCK-8) assay (Figure 5). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 °C.
Sequence Fragment: Leu133-Leu411
Tag: N-terminal His tag and SUMO tag

Human

Unconjugated

Calculated MW: 45.9 kDa  Observed MW (SDS-PAGE): 46 kDa

Recombinant

> 95%

50 µg

100 µg

200 µg

500 µg

1 mg

Lyophilized

WB, SDS-PAGE

Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.01% Sarcosyl, 5% Trehalose.

Shipped within 5-7 working days.

Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles.

No

Q96Q04

114783

Protein LMTK3

RUO

THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Reconstitute in ddH2O to a concentration up to 0.2 mg/ml. Do not vortex.
Endotoxin Level: < 1.0 EU/µg (LAL method)
Concentration: Prior to lyophilization: 200 µg/ml