Human Interleukin 27 (IL27) Protein

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935106861
info@markelab.com
Precio
247€ (10 µg)
Protein IL27
proveedor
Abbexareference
abx655896Tested Applications
WB, SDS-PAGEreactivity
status
RUOclonality
Descripción
Human Interleukin 27 (IL27) Protein is a Recombinant Human protein expressed in E. coli.
Background
Interleukin 27 (IL27) is a heterodimeric cytokine that belongs to the IL-12 cytokine family and plays a pivotal role in regulating immune responses. IL-27 is produced primarily by antigen-presenting cells (APCs), such as dendritic cells, macrophages, and monocytes, following activation by various pathogen-associated signals. Its primary function is to modulate T cell responses, particularly by promoting the differentiation of T helper 1 (Th1) cells while simultaneously limiting excessive inflammatory responses through immune suppression. This dual role positions IL-27 as a key regulator of both pro-inflammatory and anti-inflammatory immune responses. IL-27 is composed of two distinct subunits: EBI3 (Epstein-Barr virus-induced gene 3) and p28, which together form the functional cytokine. IL-27 signals through a heterodimeric receptor composed of IL-27R (WSX-1) and gp130, activating a range of signaling pathways that control immune function, including the Janus kinase/signal transducer and activator of transcription (JAK/STAT) pathway.
Características del producto
category
Proteins and Peptides
origin
Human
host
E. coli
immunogen target
Interleukin 27 (IL27)
host
E. coli
conjugation
Unconjugated
form
Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
tested applications
WB, SDS-PAGE
size 1
10 µg
size 2
50 µg
size 3
100 µg
size 4
200 µg
size 5
500 µg
observerd MW
Molecular Weight: Calculated MW: 52.6 kDa Observed MW (SDS-PAGE): 50 kDa Concentration: Prior to lyophilization: 200 µg/ml Sequence Fragment: Phe29-Pro243 + (GGGGS)*3 + Arg21-Lys229 Tag: N-terminal His tag
expression
Recombinant
purity
> 90%
buffer
Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 5% Trehalose.
storage
Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
or code
IL27
dry ice
No
availability
Shipped within 5-7 working days.
alias
p28,IL30,IL-27,IL27A,IL-27A,IL27p28,IL-27 subunit alpha,IL-27-A,Interleukin-30,p28
note
This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.
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