Human Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Protein

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935106861
info@markelab.com
Precio
247€ (10 µg)
Protein GAPDH
proveedor
Abbexareference
abx066864Tested Applications
WB, SDS-PAGEreactivity
status
RUOclonality
Descripción
Human GAPDH is a recombinant Human protein produced in a Prokaryotic expression system (E. coli).
Background
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a multifunctional enzyme predominantly involved in glycolysis, catalyzing the oxidative phosphorylation of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate using NAD+ as a cofactor. Beyond its metabolic role, GAPDH participates in various non-metabolic processes, including transcriptional regulation, DNA repair, and cell apoptosis. Its involvement in oxidative stress response and as a target for S-nitrosylation connects it to neurodegenerative diseases such as Alzheimer's and Parkinson's. Furthermore, GAPDH can translocate to the nucleus under stress conditions, influencing apoptotic pathways.
Características del producto
category
Proteins and Peptides
origin
Human
host
E. coli
immunogen target
Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)
host
E. coli
conjugation
Unconjugated
form
Lyophilized Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/ml. Do not vortex.
tested applications
WB, SDS-PAGE
size 1
10 µg
size 2
50 µg
size 3
100 µg
size 4
200 µg
size 5
500 µg
observerd MW
Molecular Weight: Calculated MW: 19.5 kDa Observed MW (SDS-PAGE): 20 kDa Concentration: Prior to lyophilization: 200 µg/ml Sequence Fragment: Thr154-Val324 Tag: N-terminal His tag
expression
Recombinant
purity
> 97%
buffer
Prior to lyophilization: 100 mM NaHCO<sub>3</sub>, 500 mM NaCl, pH 8.3, containing 0.01% Sarcosyl, 5% Trehalose.
storage
Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
or code
GAPDH
dry ice
No
availability
Shipped within 5-7 working days.
alias
GAPDH, GAPD, G3PD, HEL-S-162eP,Peptidyl-cysteine S-nitrosylase GAPDH
uniprot id
note
This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.
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