Human Gamma-Glutamyl Hydrolase (gGH) Protein

Este producto es parte de GGH - Gamma-Glutamyl Hydrolase
Human Gamma-Glutamyl Hydrolase (gGH) Protein

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935106861

info@markelab.com

Precio

221€ (10 µg)

Protein GGH

proveedor

Abbexa

reference

abx166621

Tested Applications

WB, SDS-PAGE

reactivity

status

RUO

clonality

Descripción

Human Gamma-Glutamyl Hydrolase Protein is a recombinant Human protein expressed in E. coli.


Background

Gamma-Glutamyl Hydrolase (GGH) is an enzyme involved in folate metabolism that catalyzes the hydrolysis of polyglutamated folates into monoglutamates, which are required for folate transport and cellular uptake GGH plays a key role in regulating intracellular folate levels, which are critical for DNA synthesis, repair, and methylation processes Dysregulation of GGH activity can affect cellular proliferation and is associated with conditions such as cancer, where altered folate metabolism promotes tumor growth and progression GGH expression has been linked to resistance to antifolate chemotherapeutic agents like methotrexate, as increased GGH activity reduces the retention of polyglutamated drugs, decreasing their efficacy GGH also participates in redox regulation, as folate metabolism contributes to cellular antioxidant defense pathways Studies highlight its involvement in conditions like neural tube defects and anemia due to its critical role in folate homeostasis Emerging evidence suggests that GGH activity may serve as a biomarker for cancer therapy response and other folate-related metabolic disorders

Características del producto

category

Proteins and Peptides


origin

Human


host

E. coli


immunogen target

Gamma-Glutamyl Hydrolase (gGH)


host

E. coli


conjugation

Unconjugated


form

Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in PBS, pH 7.4. If a higher concentration is required, the product can be reconstituted directly in PBS, pH 7.4, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.


tested applications

WB, SDS-PAGE


size 1

10 µg


size 2

50 µg


size 3

100 µg


size 4

200 µg


size 5

500 µg


observerd MW

Molecular Weight: Calculated MW: 37.3 kDa Observed MW (SDS-PAGE): 37 kDa Concentration: Prior to lyophilization: 200 µg/ml Sequence Fragment: Arg25-Asp318 Tag: N-terminal His tag


expression

Recombinant


purity

> 95%


buffer

Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 1 mM DTT, 5% Trehalose and Proclin-300.


storage

Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.


or code

GGH


dry ice

No


availability

Shipped within 5-7 working days.


alias

GATD10,GH,Gamma-Glu-X carboxypeptidase


note

This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.


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reactivity

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status

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clonality

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