Human Fatty Acid Binding Protein 4, Adipocyte (FABP4) Protein

Proteins and Peptides

Fatty Acid Binding Protein 4, Adipocyte (FABP4)

293F cells

Activity: Active
Biological Activity: Fatty Acid Binding Protein 4, Adipocyte (FABP4) encodes the fatty acid binding protein found in adipocytes. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. FABPs may be involved in fatty acid uptake, transport, and metabolism. Actin Beta (ACTB) has been identified as an interactor of FABP4, thus a binding ELISA assay was conducted to detect the interaction of recombinant human FABP4 and recombinant human ACTB. Briefly, FABP4 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to ACTB-coated microplate wells and incubated for 2 h at 37°C. Wells were washed with PBST and incubated for 1 h with anti-FABP4 polyclonal antibody, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody, wells were aspirated and washed 3 times. TMB substrate solution was added and wells were incubated for 15-25 minutes at 37 °C. Finally, 50 μl stop solution was added to the wells and the absorbance was read at 450 nm immediately.
Sequence Fragment: Cys2-Ala132
Tag: N-terminal His tag

Human

Unconjugated

Calculated MW: 16.2 kDa  Observed MW (SDS-PAGE): 16 kDa

Recombinant

> 95%

50 µg

100 µg

200 µg

500 µg

1 mg

Lyophilized

WB, SDS-PAGE

Prior to lyophilization: PBS, pH 7.4, containing 0.01% Sarcosyl, 5% Trehalose.

Shipped within 5-7 working days.

Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles.

No

P15090

Protein FABP4

RUO

THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Reconstitute in ddH2O to a concentration between 0.1-1.0 mg/ml. Do not vortex.
Endotoxin Level: < 1.0 EU/µg protein (LAL method)
Concentration: Prior to lyophilization: 100 µg/ml