Human Erythropoietin (EPO) Protein

Proteins and Peptides

Erythropoietin (EPO)

293F cell

Human

Unconjugated

Molecular Weight: Calculated MW: 20.0 kDa  Observed MW: 33 kDa, 40 kDa (determined by SDS-PAGE)  Possible reasons why the actual band size differs from the predicted band size: 1. Splice variants. Alternative splicing may create different sized proteins from the same gene. 2. Relative charge. The composition of amino acids may affect the charge of the protein. 3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size. 4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form. 5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed.
Concentration: Prior to lyophilization: 200 µg/ml
Sequence Fragment: Ala28-Arg193
Tag: N-terminal His tag

Recombinant

> 92%

50 µg

100 µg

200 µg

500 µg

1 mg

Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 10 mM PBS, pH 7.6. If a higher concentration is required, the product can be reconstituted directly in 10 mM PBS, pH 7.6, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.

WB, SDS-PAGE

Prior to lyophilization: 10 mM PBS, pH 7.6, 5% trehalose.

Shipped within 5-7 working days.

Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.

No

Protein EPO

RUO

This product is for research use only.   Not for human consumption, cosmetic, therapeutic or diagnostic use.