Human Epidermal Growth Factor Receptor (EGFR) Protein (Active)

Proteins and Peptides

Epidermal Growth Factor Receptor (EGFR)

Mammalian cells

Activity: Active
Biological Activity: Biological activity was measured by binding ability in functional ELISA of EGFR protein (0.1 µg/well) with a recombinant EGFR antibody (0.007629 ng/ml - 1000 ng/ml).  Detection antibody: abx341004 - Goat anti-Human IgG Fc gamma fragment specific antibody (1/10000 dilution).  EC50: 0.6154 ng/ml - 0.7361 ng/ml.
Sequence Fragment: 25-645 AA.
Tag: N-terminal His tag and C-terminal Myc tag

Human

Unconjugated

Calculated MW: 73.6 kDa
Observed MW (SDS-PAGE): 110 kDa

Recombinant

> 97% (SDS-PAGE)

Purified by affinity chromatography using IMAC. <br> 0.2 µm filtered prior to lyophilization.

20 µg

100 µg

1 mg

Lyophilized

Prior to lyophilization: PBS, pH 7.4 containing 6% Trehalose. <br> Volume prior to lyophilization: 27 μl per vial.

Shipped within 5-15 working days.

Aliquot and store at 2-8°C for up to 1 week. Store at -20 °C or -80°C for up to 1 year. Avoid repeated freeze/thaw cycles.

No

P00533

Protein EGFR

RUO

THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/ml. It is recommended to add a final glycerol concentration of 5-50%.
Endotoxin Level: < 1.0 EU/µg as determined by LAL method.