Human Cytoplasmic Aconitate Hydratase (ACO1) Protein
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Description
Aconitase-1 is a recombinant enzyme.
Documents del producto
Product specifications
| Category | Proteins and Peptides |
| Immunogen Target | Cytoplasmic Aconitate Hydratase (ACO1) |
| Host | E. coli |
| Recommended Dilution | Optimal dilutions/concentrations should be determined by the end user. |
| Origin | Human |
| Observed MW | Concentration: 0.5 mg/ml Tag: N-terminal His tag |
| Expression | Recombinant |
| Purity | > 90% (SDS-PAGE) |
| Purification | Purified by proprietary chromatographic techniques. |
| Size 1 | 1 µg |
| Size 2 | 5 µg |
| Size 3 | 50 µg |
| Form | Liquid |
| Tested Applications | SDS-PAGE |
| Buffer | 20 mM Tris-HCl (pH 8.0), 2 mM DTT, 100 mM NaCl and 10% glycerol. |
| Availability | Shipped within 5-10 working days. |
| Storage | Store at 4 °C if entire vial will be used within 2-4 weeks. Store at -20 °C for longer periods of time. For long-term storage, it is recommended to add a carrier protein (0.1% HAS or BSA). Avoid repeated freeze/thaw cycles. |
| Dry Ice | No |
| UniProt ID | P21399 |
| Alias | IRP1,ACONS,HEL60,IREB1,IREBP,IREBP1 |
| Background | Protein ACO1 |
| Status | RUO |
| Note | This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use. |
Descripción
Aconitase 1 (ACO1) is a dual-function iron-sulfur protein located in the cytoplasm, acting as both an enzyme and an iron-responsive element-binding protein (IRP1). As an enzyme, ACO1 catalyzes the reversible isomerization of citrate to isocitrate via cis-aconitate in the tricarboxylic acid (TCA) cycle, contributing to cellular energy production. Under low iron conditions, ACO1 switches to its role as IRP1, binding to iron-responsive elements (IREs) in mRNAs to regulate the expression of genes involved in iron metabolism, such as ferritin and transferrin receptor. This functional versatility allows ACO1 to coordinate cellular energy metabolism and iron homeostasis. Dysregulation of ACO1 is implicated in disorders related to iron overload or deficiency, as well as oxidative stress-induced damage in neurodegenerative diseases.
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The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants
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