Human Complement Fragment C4a (C4a) Protein

Este producto es parte de Complement C4A (Chido/Rodgers blood group)
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221€ (10 µg)

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935106861
info@markelab.com
name
Human Complement Fragment C4a (C4a) Protein
category
Proteins and Peptides
provider
Abbexa
reference
abx166451
tested applications
WB, SDS-PAGE

Description

Human Complement Fragment C4a (C4a) Protein is a recombinant Human protein expressed in E. coli.

Documents del producto

Instrucciones
Data sheet
Descargar

Product specifications

Category
Proteins and Peptides
Immunogen Target
Complement Fragment C4a (C4a)
Host
E. coli
Origin
Human
Conjugation
Unconjugated
Observed MW
Molecular Weight: Calculated MW: 38.8 kDa
Observed MW (SDS-PAGE): 42 kDa

Possible reasons why the actual band size differs from the predicted band size:
1. Splice variants. Alternative splicing may create different sized proteins from the same gene.
2. Relative charge. The composition of amino acids may affect the charge of the protein.
3. Post-translational modification. Phosphorylation, glycoslyation, methylation etc. may affect the band size.
4. Post-translational cleavage. Many proteins are synthesised as pro-proteins, and then cleaved to give the active form.
5. Polymerisation of the target protein. Dimerisation, multimerisation etc. will increase the band size observed.
Concentration: Prior to lyophilization: 200 µg/ml
Sequence Fragment: Asn680-Arg756
Tag: N-terminal His tag and GST tag
Expression
Recombinant
Purity
> 97%
Size 1
10 µg
Size 2
50 µg
Size 3
100 µg
Size 4
200 µg
Size 5
500 µg
Form
Lyophilized To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0, though please note that this will change the overall salt concentration. The stock concentration should be between 0.1-1.0 mg/ml. Do not vortex.
Tested Applications
WB, SDS-PAGE
Buffer
Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01% Sarcosyl, 5% Trehalose and Proclin-300.
Availability
Shipped within 5-7 working days.
Storage
Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Avoid repeated freeze/thaw cycles.
Dry Ice
No
UniProt ID
P0C0L4
Gene ID
720
OMIM
120790
Alias
C4,RG,C4S,CO4,C4A2,C4A3,C4A4,C4A6,C4AD,CPAMD2,Acidic complement C4,C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2
Background
Protein C4A
Status
RUO
Note
This product is for research use only.   Not for human consumption, cosmetic, therapeutic or diagnostic use.

Descripción

Complement C4A (Chido/Rodgers blood group) is a component of the classical complement pathway, which plays a crucial role in immune defense and inflammation C4A is involved in the formation of the C3 convertase complex, which leads to the activation of the complement cascade, promoting opsonization, pathogen destruction, and inflammation The Chido and Rodgers antigens are variants of C4A that are of clinical interest due to their association with blood transfusion reactions and their use as markers in blood group systems Complement C4A, along with C4B, contributes to the body's ability to fight infections, clear immune complexes, and resolve inflammation Dysregulation of C4A has been implicated in autoimmune diseases, such as lupus, where complement activation exacerbates tissue damage and inflammation C4A also influences immune responses to cancer, where its role in immune complex clearance can impact tumor progression and metastasis Understanding C4A and its polymorphisms is crucial for improving clinical approaches to immune modulation and blood group testing

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