Human Arylsulfatase B (ARSB) Protein

Proteins and Peptides

Arylsulfatase B (ARSB)

E. coli

Activity: No enzymatic activity observed.
Biological Activity: Arylsulfatase B (ARSB) is a lysosomal enzyme belonging to the sulfatase family. ARSB hydrolyzes sulfate groups in N-Acetyl-D-galactosamine, chondriotin sulfate, and dermatan sulfate. Plasminogen Activator, Urokinase Receptor (uPAR) has been identified as an interactor of ARSB, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ARSB and recombinant human uPAR. Briefly, ARSB was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 µl were then transferred to uPAR-coated microplate wells and incubated for 2 h at 37°C. Wells were washed with PBST and incubated for 1 h with anti-ARSB polyclonal antibody, then aspirated and washed 3 times. After incubation with HRP-conjugated secondary antibody, wells were aspirated and washed 3 times. TMB substrate solution was added and wells were incubated for 15-25 minutes at 37 °C. Finally, 50 µl stop solution was added to the wells and the absorbance was read at 450 nm immediately. The binding activity of ARSB and uPAR is shown in Figure 3.
Sequence Fragment: Leu81-Met533
Tag: N-terminal His tag

Human

Unconjugated

Calculated MW: 55.2 kDa  Observed MW: 55 kDa

Recombinant

> 80%

50 µg

100 µg

200 µg

500 µg

1 mg

Lyophilized

WB, SDS-PAGE

Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.01% Sarcosyl, 5% Trehalose.

Shipped within 5-7 working days.

Store lyophilized form at 2-8°C for up to 1 month. For longer periods, store lyophilized or liquid at -80°C. Avoid repeated freeze–thaw cycles.

No

P15848

411

NP_000037.2, NM_000046.3

253200

Protein ARSB

RUO

THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Reconstitute in ddH2O to a concentration between 0.1-1.0 mg/ml. Do not vortex.
Endotoxin Level: < 1.0 EU per 1 µg (determined by the LAL method).
Concentration: Prior to lyophilization: 50 µg/ml